3D打印PLLA/HA复合组织工程骨支架及性能表征  

Performance characterization of 3D printed PLLA/HA composite tissue engineering bone scaffold

作　　者：任泽宇 姜宏[1] 石永芳[2] REN Zeyu;JIANG Hong;SHI Yongfang(School of Mechanical Engineering,Xinjiang University,Urumqi,Xinjiang 830047,China;School of Medical Engineering and Technology,Xinjiang Medical University,Urumqi,Xinjiang 830017,China)

机构地区：[1]新疆大学机械工程学院,新疆乌鲁木齐830047 [2]新疆医科大学医学工程技术学院,新疆乌鲁木齐830017

出　　处：《燕山大学学报》2022年第2期116-122,共7页Journal of Yanshan University

基　　金：新疆维吾尔自治区自然科学基金资助项目(2018D01C148)。

摘　　要：为了解决羟基磷灰石(HA)骨支架降解难的问题,本研究通过在HA骨支架中加入具有良好降解性能的聚左旋乳酸(PLLA)来调节其降解速度。采用3D打印挤出成型设备制备出PLLA/HA复合多孔骨支架,探究不同PLLA质量分数下复合组织工程骨支架抗压强度、亲水性、降解率以及细胞毒性的变化。实验结果表明:五组不同材料配比的复合骨支架中,PLLA质量分数为50%的支架亲水性及抗压强度最好,且抗压强度大于人体松质骨最低承载要求的2.5 MPa。体外模拟降解10周后,骨支架的降解失重率随PLLA质量分数的提高而加快,降解液的pH值为7.3~7.5,呈弱碱性;体外细胞毒性实验中细胞生长良好。表明通过改变骨支架中PLLA的质量分数,能够实现对支架降解速率的调节,当骨支架中PLLA质量分数为50%时,支架降解速率最快,且机械性能和生物安全性能良好,对改性组织工程骨支架的降解性能调节研究具有借鉴意义。A poly l-lactic acid(PLLA)with good degradation properties is added to the HA scaffolds to adjust the degradation rate in order to solve the problem of degradation of hydroxylapatite scaffolds.Using 3D printing extrusion molding equipment,a PLLA/HA composite porous bone scaffold is produced to explore changes in the compressive strength,hydrophilicity,degradation rate and cytotoxicity of the composite tissue engineering bone scaffold under different PLLA mass fractions.The results show that PLLA with 50%mass fraction has the best hydrophilicity and compressive strength,and the compressive strength is higher than 2.5 MPa of human cancellous bone.After 10 weeks of simulated degradation in vitro,the weight loss rate of bone scaffolds degradation is accelerated with the increase of PLLA mass fraction.The pH value of the degradation solution becomes 7.3~7.5,which is weakly alkaline.The cells grow up well in vitro cytotoxicity test.The experimental results show that the scaffold degradation rate can be adjusted by changing the mass fraction of PLLA in the bone scaffold;when the mass fraction of PLLA in the bone scaffold is 50%,the scaffold′s degradation rate is the highest,and the scaffold has good mechanical and biosafety performance.The study results provide a significant reference for the research of the degradation performance regulation of modified tissue engineering bone scaffolds.

关 键 词：3D打印 组织工程骨支架 聚左旋乳酸 纳米羟基磷灰石 降解速率 细胞毒性实验 

分 类 号：TH16[机械工程—机械制造及自动化]