黄瓜穴盘苗期枯萎病抗性鉴定方法及枯萎病胁迫下的生理响应  

Evaluation of cucumber seedling resistance to Fusarium wilt and physiological response to pathogen stress

作　　者：杨凡[1,2] 唐艳领 牛莉莉[1] 马凯[1] 王晨阳[2] 米国全[1] 余其红[3] 史宣杰 YANG Fan;TANG Yanling;NIU Lili;MA Kai;WANG Chenyang;MI Guoquan;YU Qihong;SHI Xuanjie(Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou,Henan 450002,China;Henan Agricultural University,Zhengzhou 450002,Henan,China;Agricultural Comprehensive Law Enforcement Detachment of Xiangyang City,Xiangyang 441021,Hubei,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002 [2]河南农业大学,郑州450002 [3]湖北省襄阳市农业综合执法支队,湖北襄阳441021

出　　处：《中国瓜菜》2022年第3期81-85,共5页China Cucurbits And Vegetables

基　　金：河南省科技攻关项目(212102110426);河南省农业科学院科研发展专项资金(2020YQ12);河南省博士后科研项目(001702029);河南省农业科学院基本科研业务费项目(JC011)。

摘　　要：建立黄瓜枯萎病接种方法,旨在实现快速、准确的黄瓜种质资源抗病性筛选。利用下胚轴注射、菌土、胚根和灌根接种法对病原菌接种浓度、摇菌时间等进行了黄瓜苗期枯萎病抗性鉴定。结果表明,下胚轴注射接种法优于其他3种接种法,当病原菌孢子最适接种浓度为1×10^(6)孢子·mL^(-1)、最佳菌种摇菌时间为7 d时,可以有效区分黄瓜枯萎病抗感性差异。下胚轴注射接种法适用于黄瓜种质枯萎病抗性快速高效鉴定。A fast and accurate evaluation of cucumber seedling resistance to Fusarium wilt is needed.We tested hypocotyl injection,pathogen infested soil,radicle and root inoculation in this study.Inoculum of Fusarium oxysporum f.sp.cucumerinum with different concentration and medium culture periods were used for the tests.The results showed that the hypocotyl injection was better than the other three inoculation methods.The best inoculation concentration was 1×10^(6) spores·mL^(-1) and the best pathogen culturing period was 7 d for identifying Fusarium wilt resistance in cucumber cultivars.The hypocotyl injection method is suggested for screening cucumber germplasm resistance to Fusarium oxysporum f.sp.cucumerinum.

关 键 词：黄瓜枯萎病 尖孢镰刀菌病原菌 抗病性鉴定 

分 类 号：S642.2[农业科学—蔬菜学]