医用臭氧膀胱灌注治疗大鼠膀胱炎的疗效及安全性观察  

Efficacy and safety of medical ozone bladder perfusion in the treatment of cystitis in rats

作　　者：沙可夫 廖利民[1] 刘铁军[2] 王莹[2] 于春虎[2] 曲文龙 SHA Kefu;LIAO Limin;LIU Tiejun;WANG Ying;YU Chunhu;QU Wenlong(School of Rehabilitation Medicine,Capital Medical University,Department of Urology,China Rehabilitation Research Center,Beijing 100068,China;不详)

机构地区：[1]首都医科大学康复医学院中国康复研究中心泌尿外科,北京100068 [2]首都医科大学附属北京康复医院泌尿外科

出　　处：《山东医药》2022年第5期43-47,共5页Shandong Medical Journal

基　　金：北京市医院管理局临床医学发展专项经费资助项目(XYLS201839)。

摘　　要：目的观察医用臭氧膀胱灌注治疗大鼠膀胱炎的效果、安全性,并从氧化应激、炎症反应角度探讨其机制。方法选取96只同月龄雌性健康Wistar大鼠,按随机数表法取72只分为正常组(Ⅰa、Ⅰb、Ⅰc亚组各12只)和治疗组(Ⅱa、Ⅱb、Ⅱc亚组各12只),另24只分为对照组和模型组各12只。模型组和治疗组均经尿道灌注大肠杆菌悬浮液进行膀胱炎造模,正常组和对照组不进行造模处理。正常组的Ⅰa、Ⅰb、Ⅰc亚组和治疗组Ⅱa、Ⅱb、Ⅱc亚组分别以10、20、30μg/mL医用臭氧水1 mL行膀胱灌注且保留30 min,模型组和对照组以等量生理盐水行膀胱灌注,各组均连续灌注7 d。模型组和治疗组干预前后收集尿液,根据尿液细菌计数下降情况评价治疗效果;干预后模型组、治疗组与对照组同时行尿流动力学检查,处死大鼠后ELISA法检测膀胱肌层组织中的肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)。将正常组大鼠处死后取膀胱组织并行HE染色,以评价医用臭氧水浓度使用安全性。结果治疗组Ⅱa、Ⅱb、Ⅱc总有效率依次增高且均高于模型组(P均<0.05),但各亚组间差异均无统计学意义。治疗组内Ⅱa、Ⅱb、Ⅱc排尿间隔及Ⅱb、Ⅱc膀胱容量逐渐升高,且均高于模型组,但均低于对照组(P均<0.05)。治疗组内Ⅱa、Ⅱb、Ⅱc膀胱肌层组织TNF-α、IL-6表达量逐渐降低而SOD、GSH-PX活性逐渐增高,且TNF-α、IL-6表达量均低于模型组而SOD、GSH-PX活性均高于模型组,但TNF-α、IL-6表达量均高于对照组而SOD、GSH-PX活性均低于对照组(P均<0.05)。正常组Ⅰa、Ⅰb均未出现明显病理改变,Ⅰc中1只出现明显病理改变。结论短时间内应用医用臭氧膀胱灌注对大鼠膀胱炎均有较好的杀菌效果,并能在一定程度上减轻尿频、尿急等症状及炎症、氧化应激反应,尤以20、30μg/mL时最显著,但高浓Objective To observe the efficacy and safety of medical ozone bladder perfusion in the treatment of cystitis in rats,and to explore its mechanism from the perspectives of oxidative stress and inflammatory reaction.Methods Ninety-six healthy female Wistar rats at the same month age were selected and divided into groups according to random number table method,72 rats were divided into the normal group(n=12 inⅠa,Ⅰb andⅠc subgroups)and treatment group(n=12 inⅡa,Ⅱb andⅡc subgroups),and the other 24 rats were divided into the control group and model group(n=12 in each subgroup).Cystitis modeling was performed in both the model group and the treatment group through urethral infusion of EScherichia coli suspension,while no modeling was performed in the normal group and the control group.The rats in theⅠa,Ⅰb,Ⅰc subgroups of the normal group andⅡa,Ⅱb,Ⅱc subgroups of the treatment group were treated with 10,20,30μg/mL medical ozone water 1 mL for 30 min,respectively.The rats in the model group and the control group were treated with the same amount of normal saline.All rats were perfused continuously for 7 days.We collected the urine before and after intervention in the model group and treatment group,and evaluated the treatment effect according to the decrease of urine bacteria count.After intervention,we did urodynamics examination in the model group,the treatment group and the control group at the same time.After death,we detected the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),glutathione peroxidase(GSH-PX),and superoxide dismutase(SOD)in the bladder muscle tissues by ELISA.The rats of the normal group were sacrificed and the bladder tissues were stained with HE to evaluate the safety of medical ozone water concentration.Results The total effective rate of the treatment group was higher than that of the model group,and the difference between theⅡb andⅡc subgroups and the model group was statistically significant.The total effective rates ofⅡc,Ⅱb,Ⅱa in the treatment g

关 键 词：医用臭氧 膀胱炎 膀胱容量 浓度安全窗 安全性分析 氧化应激 炎症反应 

分 类 号：R694[医药卫生—泌尿科学]