30株牛源分枝杆菌分离株基因分型鉴定  

Genotyping and identification of 30 Mycobacterium bovine isolates from cattle

作　　者：邓可新 王晓平 宋孚洋[1,2] 严娜 张旭 邓光存[1,2] DENG Ke-xin;WANG Xiao-ping;SONG Fu-yang;YAN Na;ZHANG Xu;DENG Guang-cun(Ningxia University,Yinchuan 750021,China;Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China,Yinchuan 750021,China;The Fourth People’s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750021,China)

机构地区：[1]宁夏大学,银川750021 [2]西部特种生物资源保护与利用教育部重点实验室,银川750021 [3]宁夏回族自治区第四人民医院,银川750021

出　　处：《中国人兽共患病学报》2022年第3期196-202,共7页Chinese Journal of Zoonoses

基　　金：科技创新领军人才培养项目(No.KJT2017002);国家自然科学基金项目(No.31760326)联合资助。

摘　　要：目的采用基因分型方法对30株分离自西北三省的牛源分枝杆菌分离株进行鉴定。方法从西北三省的规模化奶牛场采集结核菌素(PPD)阳性奶牛的咽拭子和鼻拭子,经分离培养后,采用16S rRNA、MPT64以及多位点PCR进行菌株分型鉴定,利用数目可变串联重复序列分析(variable-number of tandem repeats,VNTR)和分枝杆菌散在分布重复单元(Mycobacterial interspersed repetitive unit,MIRU)连用的方法对其基因型进行了分析。结果菌株分型鉴定结果表明,30株牛源临床分离株全部为分枝杆菌属,其中,12株属于结核分枝杆菌复合群,18株属于非结核分枝杆菌;12株结核分枝杆菌中10株为牛分枝杆菌,2株为人型结核分枝杆菌。MIRU-VNTR分析结果表明,12株结核分枝杆菌呈现10种VNTR基因型,4株聚集为2个基因簇。结论30株分离株均属于分枝杆菌属,且结核分枝杆菌中以牛分枝杆菌为主。Genotyping was used to identify 30 isolates of Mycobacterium bovine isolated from three provinces in Northwest China.The throat and nose swabs of tuberculin(PPD)positive cows were collected from large-scale dairy farms in three provinces of northwest China.After isolation and culture,16S rRNA,MPT64 and multi-site PCR were used for typing and identification.The genotypes were analyzed by using variable-number tandem repeats analysis and the combination of Mycobacterial interspersed repetitive unit.The results showed that all the 30 bovine clinical isolates belonged to Mycobacterium genus,of which 12 belonged to Mycobacterium tuberculosis complex group and 18 belonged to non-Mycobacterium tuberculosis complex group.Among the 12 strains of Mycobacterium tuberculosis,10 strains were Mycobacterium bovis and 2 strains were Mycobacterium.Results of MIRU-VNTR analysis showed that 12 strains of Mycobacterium tuberculosis showed 10 VNTR genotypes,and 4 strains clustered into 2 gene clusters.All the 30 isolates belonged to Mycobacterium genus,and Mycobacterium bovis was the main strain of Mycobacterium tuberculosis.

关 键 词：结核分枝杆菌 牛分枝杆菌 基因分型 数目可变串联重复序列分析技术 分枝杆菌散在分布重复单元分型 

分 类 号：R378.91[医药卫生—病原生物学]