电针结合莫沙必利对糖尿病胃轻瘫大鼠胃动力的影响  

Effect of electroacupuncture combined with mosapride on gastric motility in diabetic gastroparesis rats

作　　者：陈小燕 韩旭[1] 余芝[1] 徐斌[1] CHEN Xiao-yan;HAN Xu;YU Zhi;XU Bin(Key Laboratory of Acupuncture and Medicine Research of Ministry of Education,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China)

机构地区：[1]南京中医药大学针药结合教育部重点实验室,江苏南京210023

出　　处：《中国针灸》2022年第3期298-302,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81873238、81673883、82074532、81574071;江苏省第二批中医药领军人才项目:SLJ0225;江苏省中医学优势学科三期项目立项开放课题项目:ZYX03KF012。

摘　　要：目的:观察电针“足三里”结合莫沙必利灌胃对糖尿病胃轻瘫(DGP)模型大鼠胃排空率及胃运动的影响。方法:采用随机数字表法将68只雄性SD大鼠分为空白组(12只)和造模组(56只),造模组大鼠采用腹腔注射链脲佐菌素配合高脂高糖饮食制备DGP模型。6周后,将造模成功的造模组大鼠随机分为模型组、电针组、莫沙必利组、针药结合组,每组12只。电针组大鼠予“足三里”电针干预(疏密波,2 Hz/15 Hz,电流强度为2 mA)20 min;莫沙必利组大鼠予莫沙必利灌胃(2 mg/kg);针药结合组大鼠予电针“足三里”结合莫沙必利灌胃干预。各组干预均每日1次,干预6 d后休息1 d,连续5周。干预结束后,检测各组大鼠随机血糖、胃排空率及胃运动数据(平均胃内压、胃运动幅度、胃运动频率)。结果:与空白组比较,模型组大鼠血糖升高(P<0.001);与模型组比较,电针组、莫沙必利组、针药结合组大鼠血糖降低(P<0.001,P<0.01);与莫沙必利组比较,针药结合组大鼠血糖降低(P<0.05)。与空白组比较,模型组大鼠胃排空率、平均胃内压及胃运动幅度降低(P<0.001),胃运动频率升高(P<0.001);与模型组比较,电针组、莫沙必利组、针药结合组大鼠胃排空率、平均胃内压及胃运动幅度升高(P<0.01,P<0.05,P<0.001),胃运动频率降低(P<0.001);与电针组比较,针药结合组大鼠平均胃内压及胃运动幅度升高(P<0.001);与莫沙必利组比较,针药结合组大鼠胃排空率、平均胃内压、胃运动幅度、胃运动频率及电针组大鼠胃运动频率升高(P<0.05,P<0.001,P<0.01)。结论:电针“足三里”结合莫沙必利灌胃可改善DGP大鼠血糖及胃动力,效果优于单纯电针和莫沙必利。Objective To observe the effect of electroacupuncture(EA)at"Zusanli"(ST 36)combined with mosapride on gastric emptying rate and gastric motility in the rats with diabetic gastroparesis.Methods Using random number table method,68 male SD rats were divided into a blank group(12 rats)and a model establishment group(56 rats).In the model establishment group,the models of diabetic gastroparesis were established with intraperitoneal injection of streptozotocin combined with high-fat and high-sugar diet.Six weeks later,the successful rat models in the model establishment group were randomized into a model group,an EA group,a mosapride group and a combined treatment group,12 rats in each one.In the EA group,EA was exerted at"Zusanli"(ST 36)(disperse-dense wave,2 Hz/15 Hz in frequency,2 mA in intensity)for 20 min.In the mosapride group,mosapride was intervened with intragastric administration(2 mg/kg).In the combined treatment group,electroacupuncture at"Zusanli"(ST 36)was combined with intragastric administration of mosapride.The intervention was given once daily in each group.There was 1 day at interval after 6-day intervention,consecutively for 5 weeks.At the end of intervention,the random blood glucose,gastric emptying rate and the data of gastric motility(average intra-gastric pressure,amplitude and frequency of gastric motility)were detected.Results Compared with the blank group,blood glucose was increased in the model group(P<0.001).Blood glucose was reduced in the EA group,the mosapride group and the combined treatment group as compared with the model group separately(P<0.001,P<0.01),whereas,compared with the mosapride group,blood glucose was decreased in the combined treatment group(P<0.05).In comparison with the blank group,the gastric emptying rate,the average intra-gastric pressure and the amplitude of gastric motility were all decreased in the model group(P<0.001)and the frequency of gastric motility was increased(P<0.001).Gastric emptying rate,the average intra-gastric pressure and the amplitude of gastric m

关 键 词：糖尿病胃轻瘫 针药结合 穴 足三里 莫沙必利 胃运动 

分 类 号：R245.97[医药卫生—针灸推拿学]