沉默CD44表达对鼻咽癌细胞周期及凋亡的影响  

Effects of CD44 siRNA on Cell Cycle and Apoptosis in Nasopharyngeal Carcinoma

作　　者：苏进[1] 梅君[2] 郑洲 李云霄[1] 周海波 许新华[1] Su Jin;Mei Jun;Zheng Zhou;Li Yunxiao;Zhou Haibo;Xu Xinhua(Department of Oncology,Yichang Central People's Hospital,The First College of Clinical Medical Science,China Three Gorges University&Tumor Prevention and Treatment Center of China Three Gorges University,Yichang 443003,China;Department of Stomatology,Affiliated Hospital of Hubei Three Gorges Polytechnic,Yichang 443000,China)

机构地区：[1]三峡大学第一临床医学院[宜昌市中心人民医院]肿瘤科&三峡大学肿瘤防治中心,湖北宜昌443003 [2]湖北三峡职业技术学院附属医院口腔科,湖北宜昌443000

出　　处：《巴楚医学》2022年第1期30-33,共4页Bachu Medical Journal

基　　金：湖北省卫生厅科研项目(No:JX4B52);宜昌市科技研究与开发项目(No:A17-301-14)。

摘　　要：目的:探讨CD44基因沉默对鼻咽癌细胞周期及凋亡的影响。方法:设计及合成CD44 siRNA,转染人鼻咽癌SUNE-15-8F细胞,Real-time qPCR及Western blot评价转染效果,流式细胞仪分析各组细胞周期和凋亡情况。结果:CD44 siRNA显著下调了人鼻咽癌SUNE-15-8F细胞中CD44 mRNA与蛋白表达(P<0.05)。流式细胞仪检测结果显示siRNA组细胞中G0/G1期细胞比例明显升高,S期细胞比例显著下降,细胞凋亡率明显增加(均P<0.05)。结论:CD44 siRNA干扰载体可高效抑制鼻咽癌SUNE-15-8F细胞CD44的表达,诱导细胞阻滞在G0/G1期,促进细胞凋亡。Objective:To explore the effects of CD44 siRNA on cell cycle and apoptosis in nasopharyngeal carcinoma.Methods:CD44 siRNA fragment was designed,synthesized and transfected into human nasopharyngeal carcinoma SUNE-15-8F cells.The transfection efficiency was assessed by Real-time qPCR and Western blot.The cell cycle and apoptosis were detected by flow cytometry.Results:CD44 siRNA decreased the expression of CD44 mRNA and protein significantly in SUNE-15-8F nasopharyngeal carcinoma cells(P<0.05).The proportion of G0/G1 phase cells was obviously increased,while the proportion of S phase cells was greatly decreased,with significant elevation of apoptosis rate in the siRNA group,when compared with the control group(all P<0.05).Conclusion:CD44 siRNA interference carrier could effectively inhibit the expression of CD44 in nasopharyngeal carcinoma cell,which can block cell cycle in G0/G1 phase,and enhance cell apoptosis.

关 键 词：CD44 鼻咽恶性肿瘤 RNA干扰 细胞凋亡 细胞周期 

分 类 号：R246.5[医药卫生—针灸推拿学]