基于三苯胺的增强型Fe^(3+)荧光探针及性能  

Synthesis and Performances of Triphenylamine Based Turn-on Fluorescent Probe for Fe^(3+) by An Enhancement Pattern

作　　者：尚主业 舒丽 王月[1] 孟庆涛[1] 贾宏敏[1] 张志强[1] SHANG Zhu-ye;SHU Li;WANG Yue;MENG Qing-tao;JIA Hong-min;ZHANG Zhi-qiang(School of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China)

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《发光学报》2022年第3期453-461,共9页Chinese Journal of Luminescence

基　　金：辽宁省“百千万人才计划”;辽宁省教育厅重点项目(LJKZ0277);辽宁省教育厅科技计划(2019LNQN03,2019LNJC18)资助项目。

摘　　要：Fe^(3+)在人的许多生理过程中扮演着重要的角色,例如氧气运输、DNA合成、髓磷脂合成、线粒体呼吸、神经递质合成和代谢等。然而,过量的Fe^(3+)会诱导细胞产生·OH从而引起细胞损伤,并且还会诱导β-地中海贫血、帕金森病、癫痫和阿尔茨海默病等疾病的发生。因此,对Fe^(3+)的检测和监测具有非常重要的意义。本文设计并合成了一种以三苯胺为母体的增强型Fe^(3+)荧光探针BL,并将其应用于实际水样中Fe^(3+)的识别。结果表明,探针BL对Fe^(3+)的识别具有较高的灵敏度,其检测限低至0.165μmol·L^(-1)。同时,Fe^(3+)的加入会导致探针溶液的荧光颜色由蓝绿色变为黄色,能够达到“裸眼”识别的效果。通过高分辨率质谱证明了探针与Fe^(3+)为1∶1配位。利用密度泛函理论计算了识别Fe^(3+)前后轨道能级和电能的变化并解释了其荧光光谱现象。Fe^(3+) plays an important role in many physiological processes,such as oxygen transport,DNA synthesis,myelin synthesis,mitochondrial respiration,neurotransmitter synthesis and metabolism.However,excessive Fe^(3+) can induce cells to produce a large amount of·OH and result in cell damage,which can also cause a variety of diseases,such asβ-thalassemia,Parkinson s disease,epilepsy and Alzheimer s disease.Therefore,it is of great significance to detect and monitor Fe^(3+).In this paper,a fluorescence enhanced Fe^(3+) probe BL based on triphenylamine is designed and synthesized,and it is also applied to the recognition of Fe^(3+) in real water samples.The results show that the probe BL has a high sensitivity for Fe^(3+) recognition,and the detection limit is as low as 0.165μmol·L^(-1).At the same time,upon the addition of Fe^(3+),an obvious change in the colour of the BL solution from turquoise to yellow is observed,allowing for colorimetric detection of Fe^(3+)by the“naked eye”.The 1∶1 coordination mode between BL and Fe^(3+) is further proved by high resolution mass spectrometry.Density functional theory(DFT)is used to calculate the changes of energy level and energy of the orbital before and after coordination with Fe^(3+) and explain the phenomenon of its fluorescence spectrum.

关 键 词：三苯胺 铁离子 荧光增强型探针 裸眼 识别 

分 类 号：O482.31[理学—固体物理]