甘蔗野生近缘种遗传多样性的ISSR分析  

Genetic diversity of wild cane species revealed by ISSR markers

作　　者：徐荣 徐永艳[2] 何丽莲[3] 杨清辉[3] 张汉尧[2] 李富生[3] XU Rong;XU Yong-yan;HE Li-lian;YANG Qing-hui;ZHANG Han-yao;LI Fu-sheng(Yuxi Normal University,Yuxi,Yunnan 653100,China;Southwest Forestry University,Kunming 650224,China;Yunnan Agricultur­al University,Kunming 650201,China)

机构地区：[1]玉溪师范学院,云南玉溪653100 [2]西南林业大学,昆明650224 [3]云南农业大学,昆明650201

出　　处：《西南农业学报》2022年第2期264-270,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31960451);国家重点研发计划项目(2018YFD1000503);云南省现代农业甘蔗产业技术体系建设项目(2021);云南省高校玉溪特色农业资源发掘与利用重点实验室项目。

摘　　要：【目的】评价甘蔗野生种质资源的遗传多样性,为甘蔗抗逆定向遗传改良挖掘具有育种潜力的种质资源。【方法】对44份甘蔗野生近缘种材料按照割手密种内、斑茅种内以及种间3个层次运用ISSR进行遗传多样性分析。【结果】44个种间材料利用13条ISSR引物扩增,获得261条条带,其中259条为多态性条带、占比为99%;种间多态性依次为甘蔗属割手密种>甘蔗属斑茅种>蔗茅属蔗茅种>芒属五节茅种>金茅属金茅种>河八王属河八王种;44份材料遗传相似系数为0.125~0.710,UPGMA聚类结果基本同形态学分类一致,12个斑茅种样品在0.805处聚合,斑茅和割手密在0.775处聚合,采自西藏察隅的编号A30、A32蔗茅关系密切,芒属、蔗茅属、河八王属的亲缘关系更近,与甘蔗属材料在0.73处聚合。【结论】甘蔗野生种质资源具有较高的遗传多样性,为甘蔗种质资源创新、遗传育种提供了种质材料和理论依据。[Objective]The purpose of the present paper is to evaluate the genetic diversity of wild sugarcane germplasm resources,and to explore germplasm resources with breeding potential for directional genetic improvement of sugarcane stress resistance.[Method]In the study,the genetic diversity of 44 wild relatives materials divided into three levels according to inter-species,Saccharum spontaneum,S.arundina-ceus were analyzed by ISSR molecular markers.[Result]Using 13 ISSR primers screened in 44 inter-species materials,a total of 261 DNA fragments were amplified,among which 259(99%)were polymorphic;Interspecies polymorphism:Saccharum spontaneum>Saccharum arundinaceus>Erianthus fulvus>Miscanthus floridulus>Eulalia speciosa>Narenga porphyrocoma;The genetic similarity coefficient a-mong 44 inter-species materials varied from 0.125 to 0.710.The UPGMA clustering results of the 44 interspecies materials were basically consistent with the morphological classification.The 12 S.arundinaceus materials were clustered together in 0.815;S.spontaneum and S.arundinaceus materials were close cluster together in 0.75,Narenga;Miscanthus,Erianthus materials were closer than others clustering in 0.73 with Saccharum materials.[Conclusion]The study showed that sugarcane wild germplasm resources had high genetic diversity,which could provide germplasm materials and theoretical basis for sugarcane genetic breeding.

关 键 词：甘蔗 遗传多样性 亲缘关系 野生近缘种 ISSR 

分 类 号：S566[农业科学—作物学]