基于COI基因鉴定云南省43种蝇及其遗传关系分析  

COI gene-based identification of 43 flies of Yunnan province?China and analyses of their genetic relationship

作　　者：邵仕芳 何鹏飞[1] 吴毅歆[1] 何月秋 SHAO Shi-fang;HE Peng-fei;WU Yi-xin;HE Yue-qiu(Yunnan Agricultural University,Kunming 650201,China;National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains,Kunming 650217,China)

机构地区：[1]云南农业大学,昆明650201 [2]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650217

出　　处：《西南农业学报》2022年第2期373-379,共7页Southwest China Journal of Agricultural Sciences

基　　金：云高新产业发展项目(201502)。

摘　　要：【目的】探讨DNA条形码技术应用于鉴定云南省蝇类的可行性,并初步建立云南省蝇类基因数据库。【方法】采用微生物诱剂在云南省河口县、大理市、剑川县、蒙自市和元江县诱捕双翅目昆虫,并以COI基因序列鉴定和比较科、属、种分类单元遗传距离。【结果】云南5地共诱捕到果蝇科、实蝇科、丽蝇科、厕蝇科、蝇科、麻蝇科和花蝇科等7科21属43种昆虫,它们的COI基因序列种内进化分歧均数在0.002~0.098,种间进化分歧数平均为0.156,同一科的物种大部分能聚集在一起,但也有与形态分类不一致的情况;果蝇科、实蝇科、丽蝇科、厕蝇科、蝇科、麻蝇科和花蝇科等7科的种间平均遗传距离分别是0.151、0.225、0.123、0.143、0.158、0.158、0.103,种内遗传距离分别是:0.006、0.025、0.012、0.013、0.011、0.006、0.01。7科种间遗传距离分别是种内遗传距离的25.2、10.2、10.2、11.0、14.4、27.3、10.3倍。【结论】COI基因为基础的DNA条形码技术可作为形态学鉴定的补充手段用于云南省常见蝇类的鉴定。[Objective]The present paper aims to explore the feasibility of DNA barcoding applied to the identification of flies in Yunnan province,China,and to set up DNA database for flies in Yunnan.[Method]Diptera insects trapped by microbial attractants in Hekou county,Dali city,Jianchuan county,Mengzi city and Yuanjiang county of Yunnan province were identified by COI gene sequence,and their genetic distances of families,genera and species taxa were compared.[Result]Forty-three species of insects belonging to 21 genera,7 families including Drosophilidae,Tephritidae,Calliphoridae,Fanniidae,Muscidae,Sarcophgidae and Anthomyiidae,were collected in the 5 places in Yunnan.Intraspecific divergence ranged between 0.002 and 0.098,interspecific divergence average 0.156.Phylogenetic tree showed that most of the same species gathered closely although some species were not in line with their morphology based on their sequences data.The average genetic distances among species of Drosophilidae,Tephritidae,Calliphoridae,Fanniidae,Muscidae,Sarcophgidae and Anthomyiidae were 0.151,0.225,0.123,0.143,0.158,0.158 and 0.103,respectively.The intraspecific genetic distances were 0.006,0.025,0.012,0.013,0.011,0.006 and 0.01,respectively.The genetic distances among 7 families were 25.2,10.2,10.2,11.0,14.4,27.3 and 10.3 times of intraspecific genetic distances,respectively.[Conclusion]DNA barcoding based on COI gene can be used as a complementary means for morphological identification of some common flies in Yunnan province.

关 键 词：双翅目 物种鉴定 果蝇 实蝇 丽蝇 

分 类 号：Q969[生物学—昆虫学]