植物染色体倍性鉴定方法及其在桑树研究中的应用  

Identification Method of Plant Ploidy Level and its Application in Mulberry Research

作　　者：陆晓媚 林强 唐燕梅 曾燕蓉 刘丹 李韬 朱光书 张朝华 邱长玉 LU Xiao-mei;LIN Qiang;TANG Yan-mei;ZENG Yan-rong;LIU Dan;LI Tao;ZHU Guang-shu;ZHANG Chao-hua;QIU Chang-yu(Sericulture Technology Extension Station of Guangxi Zhuang Autonomous Region,Nanning 530007,China)

机构地区：[1]广西壮族自治区蚕业技术推广站,南宁530007

出　　处：《蚕学通讯》2022年第1期30-36,共7页Newsletter of Sericultural Science

基　　金：财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-18);广西重点研发计划(桂科AB18221066)。

摘　　要：植物染色体倍性鉴定是种质资源评价和多倍体育种的一项基础性工作。本文综述了植物染色体倍性鉴定的常用方法,包括染色体计数法、植株形态指标鉴定法、细胞形态学鉴定法、流式细胞术鉴定法、分子标记鉴定法、同工酶鉴定法和生理生化指标鉴定法,并概述了上述方法在桑树种质资源研究及多倍体育种中的应用,同时针对各种鉴定方法存在的优点和缺点展开讨论,以期为桑树种质资源的创新与育种材料发掘提供参考。Plant chromosome ploidy identification is a basic work of germplasm resources evaluation and polyploid breeding.In this paper,the common methods of plant ploidy level identification were reviewed,including chromosome counting,plant morphological index identification,cell morphological identification,flow cytometry identification,molecular marker identification,isozyme identification and physiological and biochemical index identification.The applications of above methods in mulberry germplasm resources and polyploid breeding were summarized,and the advantages and disadvantages of each identification method were discussed,so as to provide reference for the innovation and development of mulberry germplasm resources.

关 键 词：植物染色体 倍性鉴定方法 种质资源 多倍体育种 桑树 

分 类 号：S888.4[农业科学—特种经济动物饲养]