miR-199a-5p对心脏缺血再灌注细胞模型细胞活力和细胞凋亡的影响及可能机制探究  

Effect of MiR-199a-5p on Cell Viability and Apoptosis of Cardiac Ischemia-Reperfusion Cell Model and its Possible Mechanism

作　　者：郭玉冰 王皓 谷云飞[1] GUO Yu-bin;WANG Hao;GU Yun-fei(Luoyang Central Hospital,Luoyang,Henan,471000,China)

机构地区：[1]洛阳市中心医院,河南洛阳471000

出　　处：《中国血液流变学杂志》2021年第4期458-462,共5页Chinese Journal of Hemorheology

摘　　要：目的探究微小RNA-199a-5(microRNA-199a-5p,miR-199a-5p)对心脏缺血再灌注细胞模型细胞活力和细胞凋亡的影响及可能机制。方法利用H9C2细胞株建立氧糖剥夺再灌注模型,模拟心肌细胞缺血再灌注。利用miR-199a-5p inhibitor抑制H9C2细胞中miR-199a-5p的表达,细胞分组为空白对照组、模型组、miR-199a-5p inhibitor组。观察空白对照组、模型组细胞中miR-199a-5p的表达水平,比较空白对照组、模型组、miR-199a-5p inhibitor组细胞活力、凋亡能力及缺氧诱导因子-1α(HIF-1α)、糖原合成激酶3β(GSK3β)、p-GSK3β蛋白表达水平。结果模型组H9C2细胞中miR-199a-5p表达水平显著高于空白对照组(P<0.05)。模型组H9C2细胞OD值显著低于空白对照组,凋亡率显著高于空白对照组(P<0.05)。miR-199a-5p inhibitor组H9C2细胞OD值显著高于模型组,凋亡率显著低于模型组(P<0.05)。模型组H9C2细胞HIF-1α、p-GSK3β蛋白表达水平均显著低于空白对照组。miR-199a-5p inhibitor组H9C2细胞HIF-1α、p-GSK3β蛋白表达水平均显著高于模型组(P<0.05)。结论miR-199a-5p在心脏缺血再灌注细胞模型中的表达水平增加,敲低其表达水平后可抑制细胞活力并促进细胞凋亡,其作用机制可能是通过上调HIF-1α、p-GSK3β表达水平实现的。Objective To investigate the effect of microRNA-199a-5p(miR-199a-5p)on cell viability and apoptosis of cardiac ischemia-reperfusion cell model and its possible mechanism.Methods Oxygen and glucose deprivation reperfusion model was established with H9C2 cell line to simulate myocardial ischemia-reperfusion.miR-199a-5p inhibitor was used to inhibit the expression of miR-199a-5p in H9C2 cells,and the cells were divided into blank control group,model group,and miR-199a-5p inhibitor group.The expression levels of miR-199a-5p in blank control group and model group were observed.The cell viability,apoptosis ability and expression levels of HIF-1α,GSK3βand p-GSK3βin blank control group,model group and miR-199a-5p inhibitor group were compared.Results The expression level of miR-199a-5p in H9C2 cells of model group was significantly higher than that of blank control group(P<0.05).The OD value of H9C2 cells in the model group was significantly lower than that in the blank control group,and the apoptosis rate was significantly higher than that in the blank control group(P<0.05).The OD value of H9C2 cells in miR-199a-5p inhibitor group was significantly higher than that in model group,and the apoptosis rate was significantly lower than that in model group(P<0.05).The expression levels of HIF-1αand p-GSK3βin H9C2 cells of model group were significantly lower than those of blank control group.The expression levels of HIF-1αand p-GSK3βin H9C2 cells in miR-199a-5p inhibitor group were significantly higher than those in model group(P<0.05).Conclusion The expression level of miR-199a-5p is increased in the cell model of cardiac ischemia-reperfusion.Knocking down the expression level of miR-199a-5p can inhibit cell viability and promote cell apoptosis.Its mechanism may be realized through up-regulating the expression levels of HIF-1αand p-GSK3β.

关 键 词：miR-199a-5p 心脏缺血再灌注 HIF-1Α p-GSK3β 

分 类 号：R541[医药卫生—心血管疾病]