Determination of trophic levels of marine fish in the Yellow Sea and northern East China Sea using nitrogen stable isotope (δ^(15)N) analysis of otoliths  

作　　者：Huaiyu BAI Yukun WANG Tingting ZHANG Fangqun DAI Lingfeng HUANG Yao SUN 

机构地区：[1]College of the Environment and Ecology,Xiamen University,Xiamen 361102,China [2]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China [3]Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,Xiamen University,Xiamen 361005,China [4]Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China

出　　处：《Journal of Oceanology and Limnology》2022年第2期634-642,共9页海洋湖沼学报（英文）

基　　金：Supported by the National Key Basic Research Development Program of China(973 Program)(No.2015CB453303);the National Science Foundation for Young Scientists of China(No.41606195)。

摘　　要：Fish otolithδ^(15) N(δ^(15) N_(oto))is a demonstrated source of information of dietary history for marine fi sh as it is available iN_(oto)lith archives and sedimentary deposits unlike white muscle tissue(WMT).WMT and stomach content data are insufficient for trophic level(TL)data of past fi shes which is important for the changes of marine fi shery resources over long time scales.To determine the correlation betweenδ^(15) N_(oto) and fi sh WMTδ^(15) N(δ^(15) N_(wmt))and the feasibility of usingδ^(15) N_(oto) in characterizing the TLs of marine fi shes,we conducted nitrogen stable isotope analysis(SIA)in the otolith and WMT of 36 marine fi sh species sampled from the Yellow Sea and northern East China Sea in 2011-2014.Bothδ^(15) N_(oto) andδ^(15) N_(wmt) were analyzed using an elemental analyzer coupled with an isotope ratio mass spectrometer(EA-IRMS).Multiple otoliths were combined to make each otolith measurement and were analyzed as-is without a carbonate dissolution pre-processing step.δ^(15) N_(oto) andδ^(15) N_(wmt) comparisons for species in the Yellow Sea and northern East China Sea are currently lacking and would be helpful for both regional studies and for increasing the number of species for whichδ^(15) N_(oto) andδ^(15) N_(wmt) have been compared.Additionally,to determine the relative accuracy of trophic level calculated usingδ^(15) N_(oto),we compared TL calculated fromδ^(15) N_(oto) to traditional trophic level metrics calculated usingδ^(15) N_(wmt).The results showed a positive and highly signifi cant correlation(R=0.780,P<0.001)betweenδ^(15) N_(oto) andδ^(15) N_(wmt).Trophic level estimation using WMT(TL wmt)and otolith(TL oto)showed congruence in our study,which is not entirely surprising given thatδ^(15) N_(oto) was regressed againstδ^(15) N_(wmt) and the resulting regression coefficient was used to convertδ^(15) N_(oto) toδ^(15) N_(wmt) prior to calculating TL oto.This conversion was required in order to be consistent with previousδ^(15) N_(wmt)-based calculations of TL fo

关 键 词：stable isotope analysis δ^(15)N OTOLITH trophic level 

分 类 号：Q958.8[生物学—动物学]