Transcriptome of hepatopancreas in kuruma shrimp Marsupenaeus japonicus under low-salinity stress  

作　　者：Tingjun CHEN Zhimin LI Jianyong LIU Caifeng LIANG Le YUAN 

机构地区：[1]Guangdong Ocean University,Zhanjiang 524088,China [2]Guangdong Provincial Shrimp Breeding and Culture Laboratory,Guangdong Ocean University,Zhanjiang 524088,China

出　　处：《Journal of Oceanology and Limnology》2022年第2期745-765,共21页海洋湖沼学报（英文）

基　　金：Supported by the Project of 2019 Annual Guangdong Provincial Special Financial Fund of Nanhai Economic Shrimp Breeding and Culture Laboratory(No.2319412525);the Competitive Allocation Project of Special Funds for Science and Technology Innovation Strategy of Guangdong Province(No.2018A04007);the Competitive Distribution Project of Special Funds for Science and Technology Development in Zhanjiang City(No.2018A01013)。

摘　　要：The kuruma shrimp Marsupenaeus japonicus is one of the most commercially important shrimp species in the world.Low salinity would aff ect the penetration and immunity,and even led to its death of the shrimp.However,little is known about the molecular mechanism of the effect.Therefore,hepatopancreas of M.japonicus reared under low-salinity stress for 6,12,24,48,and 96 h was analyzed and the results were compared with that of the control group using transcriptomics.After removing reads containing adapters,88890960-1051300444 clean reads were generated from 10 libraries in the control group and experimental group.Compared with the control group,811,589,1095,745,and 875 diff erentially expressed genes were obtained in the five treatment groups.The N50 and N90 lengths of the transcripts were 1746 bp and 436 bp,respectively.The top 20 gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways associated with the diff erentially expressed genes were related mainly to osmotic regulation(ion exchange,lipid metabolism and carbohydrate metabolism),immune regulation(cellular and humoral immunity),chitin metabolism,and related functions.The diff erential expression patterns of nine randomly selected genes were confi rmed by quantitative real-time PCR.This is the fi rst report of osmotic regulation-related genes that are diff erentially expressed under low-salinity stress in the hepatopancreas of M.japonicus.Furthermore,we found that M.japonicus initiated its own immune regulation under low-salinity stress.These results will help elucidating the mechanism of osmotic regulation and immune responses in this shrimp species.

关 键 词：Marsupenaeus japonicus TRANSCRIPTOME osmotic regulation immune responses 

分 类 号：S917.4[农业科学—水产科学]