一种识别Cu^(2+)的罗丹明衍生物的合成及其性质研究  

Synthesis and properties of the Rhodamine derivative to detect Cu^(2+)

作　　者：张杰 戴红亮 郑琦君 覃于鑫 白翠冰 张琳 李瑞乾 乔瑞 苗慧 ZHANG Jie;DAI Hongliang;ZHENG Qijun;QIN Yuxin;BAI Cuibing;ZHANG Lin;LI Ruiqian;QIAO Rui;MIAO Hui(School of Chemistry and Materials Engineering,Fuyang Normal University,Fuyang Anhui 236041,China)

机构地区：[1]阜阳师范大学化学与材料工程学院,安徽阜阳236037

出　　处：《阜阳师范大学学报（自然科学版）》2022年第1期47-54,F0002,共9页Journal of Fuyang Normal University:Natural Science

基　　金：安徽省高校自然科学研究项目(KJ2019ZD38);安徽省自然科学基金项目(1908085QB78);阜阳师范大学-阜阳市政府市校合作项目(SXHZ202002);大学生创新训练项目(202010371029,S202010371050)资助。

摘　　要：设计合成得到了一种罗丹明衍生物LX,通过^(1)H NMR和^(13)C NMR确定了化合物LX的结构。根据吸收光谱、发射光谱与红外光谱,研究了化合物LX与Cu^(2+)之间的相互作用。化合物LX对Cu^(2+)的最低检出限为1.06×10^(-8)M,实现了有效检测水溶液中的Cu^(2+)。通过荧光成像方法,化合物LX还可以检测线虫体内的Cu^(2+)。A rhodamine derivative LX was designed and synthesized,and the structure of the compound LX was confirmed by^(1)H NMR and^(13)C NMR.According to the ultraviolet and fluorescence spectrum,the stoichiometric ratio of the interaction between the compound LX and Cu^(2+) was studied.In addition,the lowest detection limit of LX for Cu^(2+) was calculated to be 1.06×10^(-8)M and could detect Cu^(2+) in water well.And the compound LX was successfully applied for biological imaging in caenorhabditis elegans.

关 键 词：荧光探针 生物成像 Cu^(2+) 

分 类 号：Q-337[生物学]