28%三环唑·嘧菌酯悬浮剂的高效液相色谱分析  

An analysis method of tricyclazole·azoxystrobin 28%SC by HPLC

作　　者：孙钰洁[1] 颜明[1] SUN Yujie;YAN Ming(Jiangsu Province Agricultural Products Quality Inspection&Test Center,Nanjing 210036,China)

机构地区：[1]江苏省农产品质量检验测试中心,江苏南京210036

出　　处：《世界农药》2022年第3期57-60,共4页World Pesticide

摘　　要：为了实现产品生产与质量控制,建立了同时测定28%三环唑·嘧菌酯悬浮剂中三环唑和嘧菌酯的高效液相色谱分析法。该方法以甲醇和水做流动相,采用梯度洗脱程序,以Zorbax SB-C18为填料的不锈钢柱和紫外检测器,在波长250 nm对试样中的三环唑和嘧菌酯进行高效液相色谱分离和分析,以外标法定量。结果表明,三环唑为0.00040~0.00200 g/mL、嘧菌酯为0.00022~0.00160 g/mL时,线性相关系数分别为0.9995和0.9996,标准偏差分别为0.0659和0.0562,变异系数分别为0.33%和0.69%,平均回收率分别为100.29%和99.77%。该方法具有简便、快速、准确度高等特点,可以满足三环唑和嘧菌酯的定性和定量分析。A HPLC method was developed for the simultaneous quantitative determination of tricyclazole and azoxystrobin in the formulation product of tricyclazole·azoxystrobin 28%SC.The sample was extracted by methanol,then separated and analyzed quantitatively on Zorbax SB-C18 column and UV detector at 250 nm,using methanol and water as mobile phase by reverse phase HPLC.The results showed,in the rang of 0.00040~0.00200 g/mL and 0.00022~0.00160 g/mL,the linear correlation coefficients of tricyclazole and azoxystrobin were 0.9995 and 0.9996,the standard deviation were 0.0659 and 0.0562,the variation coefficients were 0.33%and 0.69%,the average recovery rates were 100.29%and 99.77%,respectively.The method is simple,quick,accurate,and suitable for the analysis of tricyclazole and azoxystrobin in the mix formulation products.

关 键 词：三环唑 嘧菌酯 高效液相色谱 分析 

分 类 号：TQ450.7[化学工程—农药化工]