甘肃省党参根腐病菌的分子生物学鉴定及区域分布  

Molecular biological identification and regional distribution of pathogens causing root rot of Codonopsis pilosula in Gansu province

作　　者：徐雪芬 李惠霞 倪春辉 李文豪 李焕宇 陈垣[2] 胡芳弟[3] XU Xuefen;LI Huixia;NI Chunhui;LI Wenhao;LI Huanyu;CHEN Yuan;HU Fangdi(College of Plant Protection,Gansu Agricultural University,Engineering Laboratory for Biological Control of Crop Diseases and Pests of Gansu Province,Lanzhou730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou730070,China;College of Pharmacy,Lanzhou University,Lanzhou730030,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070 [2]甘肃农业大学农学院,兰州730070 [3]兰州大学药学院,兰州730030

出　　处：《植物保护》2022年第2期130-138,共9页Plant Protection

基　　金：国家重点研发计划(2018YFC1706301);甘肃省现代农业(中药材)产业技术体系(GARS-ZYC-4)。

摘　　要：为明确甘肃省党参根腐病病原菌种类、优势种及病原菌种群在不同区域的分布差异,采用组织分离法对甘肃省陇西、渭源、临洮、岷县和宕昌等5个党参主产县的病样进行分离纯化和柯赫氏法则验证,通过rDNA-ITS、TEF-1α序列聚类分析鉴定不同区域病原菌种类及分离频率。结果发现,引起甘肃省党参根腐病的病原菌主要为镰孢属Fusarium真菌,分离频率为72.66%,包括尖镰孢F.oxysporum、锐顶镰孢F.acuminatum、茄镰孢F.solani、木贼镰孢F.equiseti和燕麦镰孢F.avenaceum等9个种,其中尖镰孢和锐顶镰孢为优势种,分离频率分别为41.80%和16.41%。5个主产县党参根腐病病原种类及其分离频率各不相同,但优势属均为镰孢属,优势种均为尖镰孢。其中渭源县分离的病原菌种类最多,分离到镰孢属的6个种;其次为岷县,分离到镰孢属的5个种;临洮县分离的病原菌种类最少。部分镰孢菌在甘肃省党参产区为首次发现。To identify the species,dominant species and regional distribution of the root rot pathogens of Codonopsis pilosula in Gansu province,the tissue isolation method was used to isolate and purify the pathogens from diseased samples collected from five main producing counties in Gansu province,including Longxi county,Weiyuan county,Lintao county,Minxian county and Tanchang county,and verification test was carried out according to the Koch’s rule in this study.The pathogen species and isolation frequencies in different regions were determined by sequence cluster analysis of rDNA-ITS and TEF-1α.The results showed that Fusarium spp.was the main pathogen of C.pilosula root rot in Gansu province;its isolation frequency was 72.66%,including F.oxysporum,F.acuminatum,F.solani,F.equiseti and F.avenaceum.F.oxysporum and F.acuminatum were the dominant species,with an isolation frequency of 41.80%and 16.41%,respectively.The pathogen species and isolation frequency of C.pilosula root rot were different in five main producing counties,but the dominant genus and the dominant species in all regions were Fusarium and F.oxysporum.Among the counties,most species of pathogens were found in Weiyuan county,including six species of Fusarium,followed by Minxian county(five species),and the least species of pathogens were found in Lintao county.Some Fusarium species were found for the first time in C.pilosula planting areas in Gansu province.

关 键 词：党参 党参根腐病 RDNA-ITS TEF-1α 地域性差异 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]