甘肃陇东大豆孢囊线虫田间侵染动态  

Field infection dynamics of soybean cyst nematode in east of Gansu province

作　　者：罗宁 李惠霞 刘永刚[2] 李焕宇 杨顺义 李金章[3] 石明明 李金鸿 LUO Ning;LI Huixia;LIU Yonggang;LI Huanyu;YANG Shunyi;LI Jinzhang;SHI Mingming;LI Jinhong(College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou730070,China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou730070,China;Gansu Qingyang Agricultural Technology Promotion Center,Qingyang745000,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070 [2]甘肃省农业科学院植物保护研究所,兰州730070 [3]甘肃省庆阳市农业技术推广中心,庆阳745000

出　　处：《植物保护》2022年第2期214-219,231,共7页Plant Protection

基　　金：国家公益性行业(农业)科研专项(201503114);甘肃省科技支撑计划(1604NKCA053-2)。

摘　　要：为探讨甘肃陇东地区大豆孢囊线虫田间侵染动态,于2017年-2019年对大豆根系和根际土壤定期定点取样,采用漂浮法、贝曼漏斗法及酸性品红染色法对土样中和根组织内线虫进行分离计数,研究其繁殖数量与气温、降雨量的关系。结果表明,2017年土壤中2龄幼虫和新鲜孢囊的数量有3个明显高峰,大豆根组织内2~4龄幼虫均出现3个明显高峰,即发生3代,平均世代历期35 d。2018年-2019年土壤中2龄幼虫和新鲜孢囊均有4个明显高峰,大豆根组织内2~4龄幼虫亦出现4个明显高峰,即发生4代。第1代侵染数量最多,是主要危害世代。最后1代侵染数量极少且发育缓慢。在2017年-2019年生长季中,线虫繁殖数量与生长季平均温度具有相关性,与累积降雨量无相关性。In order to explore the infection dynamics of soybean cyst nematode in the soybean field in east of Gansu province,soil and root samples were periodically collected from one fixed soybean block from 2017 to 2019.The nematodes were separated and counted under microscope by floating,Baermann funnel and acid fuchsin staining method,and the relationship between the reproduction and temperature and humidity were investigated.The results showed that there were three distinct number peaks of 2nd stage juvenile and new cysts in the soil,and three peaks of the emergence of 2nd,3rd and 4th juveniles in soybean roots,which occurred in three generations and the average period of one generation was 35 d in 2017.From 2018 to 2019,there were four distinct number peaks of 2nd stage juvenile and new cysts in the soil and four peaks of the emergence of 2nd,3rd and 4th juveniles in soybean roots,indicating that four generations occurred.The first generation had the largest number of infections,which was the main damage period and nematodes developed slowly in last generation.During the growing season from 2017 to 2019,the number of nematodes reproduction was correlated with the average temperature,but not with the accumulated rainfall.

关 键 词：大豆 大豆孢囊线虫 侵染特征 世代循环 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]