从江田鱼肠道微生物多样性分析  

Analysis of the bacterial diversity in the Congjiang field carp’s intestine

作　　者：李小义 申晓东 张效平 杨星 关梅 王雪 LI Xiao-yi;SHEN Xiao-dong;ZHANG Xiao-ping;YANG Xing;GUAN Mei;WANG Xue(Guizhou Fisheries Research Institute,Guizhou Academy of Agricultural Sciences/Guizhou Special Aquatic Products Engineering Technology Center,Guiyang 550025,China)

机构地区：[1]贵州省农业科学院水产研究所/贵州省特种水产工程技术中心,贵阳550025

出　　处：《湖北农业科学》2022年第5期56-59,75,共5页Hubei Agricultural Sciences

基　　金：贵州省科技计划项目(黔科合支撑[2017]2537);国家自然科学基金地区基金项目(31660746);贵州省特色水产产业技术体系(GZ⁃CYTX2019-01102);国家特色淡水鱼产业技术体系贵阳综合试验站(CARS-46)。

摘　　要：对不同养殖时间点(1 d、2个月、5个月)的从江田鱼肠道细菌进行了测序分析。结果表明,RS1(1 d)和RS3(5个月)组样品肠道细菌多样性高于RS2(2个月)组。门水平分析,RS1组样品中厚壁菌门(Firmicutes,33.43%)、蓝细菌(Cyanobacteria,28.15%)、变形菌门(Proteobacteria,22.94%)和拟杆菌门(Bacteroidetes,9.79%)为主要优势菌;RS2组样品中的优势菌门为变形菌门,所占丰度为94.05%,其次为厚壁菌门(2.58%)、梭杆菌门(Fusobacteria,2.05%)和放线菌门(Actinobacteria,0.68%)。RS3组样品中优势菌门为蓝细菌(40.69%)、变形菌门(31.65%)、梭杆菌门(10.30%)和厚壁菌门(8.89%)。属水平分析,RS1组微生物多样性较高,未分类的蓝细菌(unidentified Cyanobacteria,28.11%)丰度最高,其次为Romboutsia(13.33%)、未分类的梭菌属(unidentified Clostridiales,8.37%)、鞘氨醇单胞菌(Sphingomonas,4.62%)等菌属。RS2组样品中,气单胞菌属(Aeromonas)相对丰度最高,为68.86%。RS3组样品中,未分类的蓝细菌相对丰度最高,为40.63%;其次是气单胞菌属(12.41%)、鲸杆菌属(Cetobacterium,10.20%)等菌属。研究结果表明,稻田养殖系统中从江田鱼的肠道细菌群落在不同生长阶段变化明显。The intestinal bacterial community at three different stages in Congjiang field carp(1 d,2 months,5 months)were sequenced and analyzed.The results showed that the RS1(1 d)and RS3(5 months)group had higher bacterial diversity than the RS2(2 months)group.At the phylum level,Firmicutes(33.43%),Cyanobacteria(28.15%),Proteobacteria(22.94%)and Bacteroidetes(9.79%)dominated the microbiota of the RS1.The intestinal bacterial community in RS2 was dominated by Proteobacteria(94.05%),followed by Firmicutes(2.58%),Fusobacteria(2.05%)and Actinobacteria(0.68%).The dominant bacteria in RS3 group were Cyanobacteria(40.69%),Proteobacteria(31.65%),Fusobacteria(10.30%)and Firmicutes(8.89%).At the genus level,The RS1 had high microbial diversity,with the highest abundance of unidentified Cyanobacteria(28.11%),followed by Romboutsia(13.33%),unidentified Clostridiales(8.37%)and Sphingomonas(4.62%).Aeromonas(68.86%)had the highest relative abundance in the RS2.The intestinal bacterial community in RS3 was dominated by unidentified Cyanobacteria(40.63%),followed by Aeromonas(12.41%)and Cetobacterium(10.20%).The results showed that the the intestinal bacterial community of Congjiang field carp in the paddy field culture system changed significantly at different growth stages.

关 键 词：肠道细菌 从江田鱼 多样性 高通量测序 

分 类 号：S917.4[农业科学—水产科学]