鸡毒支原体MG荧光定量PCR技术评估实验室MG核酸污染方法  

Diagnosis and control measures of chicken infectious anemia

作　　者：张承新 张鹏 彭晓艳 王海兰 曲秀娟 姜皓 卢艳慧 李新 郭龙宗 李玉燕 ZHANG Chengxin;ZHANG Peng;PENG Xiaoyan;WANG Hailan;QU Xiujuan;JIANG Hao;LU Yanhui;LI Xin;GUO Longzong;LI Yuyan(Shandong Yisheng Animal and Poultry Breeding Company,Shandong Yantai 264680,China)

机构地区：[1]山东益生种畜禽股份有限公司,山东烟台264680

出　　处：《家禽科学》2022年第3期60-62,共3页Poultry Science

基　　金：“财政部和农业农村部:国家现代农业产业技术体系资助”(CARS-41);山东省重点研发计划,项目编号:2021CXGC010805

摘　　要：为了评估鸡毒支原体(MG)非免疫鸡群的MG感染,常采用MG荧光定量PCR技术进行监测;而在鸡群的MG净化监控前,需要对实验室进行MG核酸污染评估,无污染后才能进行后续的净化检测工作。本实验采用MG荧光定量PCR技术评估实验室各区域的MG核酸污染情况,如果存在核酸污染,需要采取一系列的消毒措施,直至实验室MG核酸污染监控为阴性。In order to evaluate the Mg infection of non immune chickens with Mycoplasma gallisepticum(mg), Mg fluorescence quantitative PCR is often used for monitoring. Before mg purification monitoring of chickens, it is necessary to evaluate the nucleic acid pollution of Mg in the laboratory, and the subsequent purification and detection can be carried out only after there is no pollution. In this experiment, Mg fluorescence quantitative PCR technology is used to evaluate the Mg nucleic acid pollution in various areas of the laboratory. If there is nucleic acid pollution, a series of disinfection measures need to be taken until the monitoring of Mg nucleic acid pollution in the laboratory is negative.

关 键 词：鸡毒支原体 荧光定量PCR 核酸污染 

分 类 号：S858.31[农业科学—临床兽医学]