不同光质对人参愈伤组织生长及皂苷含量的影响  

Effects of Different Light Qualities on Growth and Ginsenoside Contents in Callus of Panax ginseng

作　　者：任跃英[2] 牛晨 王京京 杨鹤[1,2] 许永华[1,2] 刘志[2] REN Yue-ying;NIU Chen;WANG Jing-jing;YANG He;XU Yong-hua;LIU Zhi(State Local Joint Engnieering Research Center of Ginseng Breeding and Application,Jilin Agricultural University,Changchun 130118,China;College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学人参新品种选育与开发国家地方联合工程研究中心,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《光谱学与光谱分析》2022年第4期1318-1322,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31770378)资助。

摘　　要：人参主要依靠大田栽培,耗时长,利用植物组织培养技术不仅可以缩短育种年限,还可以用来生产次生代谢产物。在组织培养中,光质对于药用植物次生代谢产物的影响受到了人们广泛关注。以人参愈伤组织为试材,采用超高效液相色谱法,研究了不同光质(包括红光、红蓝光、蓝光、绿光、黄绿光)对人参愈伤组织生长状态、总皂苷及9种皂苷单体Rg1,Re,Rf,Ro,Rb1,Rc,Rb2,Rb3,Rd含量的影响。结果表明:绿光加速人参愈伤组织老化,促进次生代谢产物的积累,而蓝光对人参愈伤组织生长有促进作用;红光和绿光对总皂苷作用不明显,且蓝光、红蓝光(1∶1)、黄绿光(1∶1)对人参皂苷转化与合成起到明显的抑制作用;与对照组相比,绿光处理后Rg1、Rf含量均偏高,其含量分别为4.063和1.194 mg·g^(-1),对Rg1、Rf人参皂苷单体含量有促进作用。表明不同光质对人参愈伤组织生长及皂苷含量有不同的影响,可以通过绿光处理来获得人参单体皂苷Rg1和Rf。该研究旨在探究光质对人参愈伤组织生理生化的影响,提高人参皂苷含量,为工业化生产提供理论依据。Ginseng mainly relies on field cultivation,which takes a long time.The use of plant tissue culture technology can not only shorten the breeding period,but also can be directly used to produce secondary metabolites.In plant tissue culture,the influence of light quality on the secondary metabolites of medicinal plants has attracted widespread attention.In our work,ginseng callus was used as the experimental material,and ultra-high performance liquid chromatography was used to study the effects of different light qualities(including red light,red-blue light,blue light,green light,yellow-green light) on the total saponins and the contents of 9 saponins monomers Rg1,Re,Rf,Ro,Rb1,Rc,Rb2,Rb3,Rd.The results show that green light accelerates the aging of ginseng callus and promotes the accumulation of secondary metabolites,while blue light can promote the growth of ginseng callus;red light and green light have no obvious effect on total saponins,and blue light,red-blue light(1∶1),yellow-green light(1∶1) have obvious inhibitory effects on the conversion and synthesis of ginsenosides;compared with the control group,the contents of Rg1 and Rf were higher under green light treatment,and the contents were 4.063 and 1.194 mg·g^(-1),respectively,the green light also promoted the content of Rg1 and Rf ginsenoside monomers.This experiment shows that different light qualities have different effects on the growth of ginseng callus and the content of saponins.Green light treatment is beneficial to obtain ginseng monomer saponins Rg1 and Rf.The purpose of this article is to explore the effects of light quality on the physiology and biochemistry of ginseng callus,increase the content of ginsenosides,and provide a theoretical basis for industrial production.

关 键 词：人参 愈伤组织 光质 人参皂苷 超高效液相色谱 

分 类 号：O657.72[理学—分析化学]