硝酸铈亚慢性暴露对大鼠神经行为功能的影响  

Effects of sub-chronic exposure to cerium nitrate on neurobehavioral functions in SD rats

作　　者：刘健新 马宇鸿 郝卫东[1] LIU Jianxin;MA Yuhong;HAO Weidong(Department of Toxicology,School of Public Health,Peking University/Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety,Beijing 100191,China)

机构地区：[1]北京大学公共卫生学院毒理学系,食品安全毒理学研究与评价北京市重点实验室,北京100191

出　　处：《癌变．畸变．突变》2022年第2期81-87,共7页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家重点研发计划项目(2017YFC1600203)。

摘　　要：目的:评估硝酸铈[Ce(NO_(3))_(3)]亚慢性(90 d)暴露对SD大鼠神经行为功能的影响,为稀土元素铈的风险评估提供科学依据。方法:选用初断乳SD大鼠,根据体质量随机分为4组,分别为正常对照组(ddH2O),Ce(NO_(3))_(3)低剂量组20 mg/(kg·d)、中剂量组100 mg/(kg·d)和高剂量组500 mg/(kg·d),每组雌雄鼠各12只。灌胃染毒90 d后进行高架十字迷宫实验、旷场实验、转棒实验和Morris水迷宫实验。通过HE染色对各组大鼠脑组织进行病理学检测。结果:与对照组相比,硝酸铈经口暴露90 d后,转棒实验结果显示硝酸铈各剂量组雌、雄大鼠的在棒时间差异无统计学意义(P>0.05);旷场实验和高架十字迷宫实验结果表明进入中央区次数、中央区停留时间、进入开臂次数百分比和进入开臂时间百分比等指标的差异均无统计学意义(P>0.05);Morris水迷宫实验结果显示4 d定位航向实验的逃避潜伏期和空间探索实验的各个指标,包括进入目标象限次数、目标象限游泳时间和穿过原平台次数等差异均无统计学意义(P>0.05)。脑组织HE染色结果也未观察到明显的中毒病理特征。结论:硝酸铈亚慢性经口暴露可能不会引起SD大鼠焦虑及损害SD大鼠的运动协调平衡能力和学习记忆能力。OBJECTIVE:To evaluate effects of sub-chronic(90 d)exposure to cerium nitrate[Ce(NO_(3))_(3)]on neurobehavioral functions in SD rats and to provide scientific basis for risk assessment of rare earth element cerium.METHODS:SD rats(PND21)were randomly divided into 4 groups(12 males and 12 females in each group)by body mass,which were control group(exposure to ddH2O)and 20,100,500 mg/(kg·d)Ce(NO_(3))_(3) exposure groups.The rats were administrated orally with Ce(NO_(3))_(3) for 90 days.Neurobehavioral functions were then evaluated by experiments such as elevated plus maze,open field,rotarod and Morris water maze.Histopathological structure of hippocampus was analyzed with HE staining.RESULTS:Compared with the control group,the exposures did not cause significant changes in time on the rotarod test.From the Open field and elevated plus maze tests,there was no statistically significant differences between groups of rats in the time spent in the center of the arena,the number of entrances into the center of the arena,the percentage of entrances into the open arms and the time spent on the open arms.In the Morris water test,the difference in the escape latencies among the three cerium nitrate exposed groups and the control group was not statistically significant,nor were the differences in various metrics of the space exploration test including the times crossing platform,swimming speed and the times required for swimming in target quadrant.Histopathological analysis showed that no pathological changes were observed in the dosing groups.CONCLUSION:Sub-chronic oral exposure to cerium nitrate did not cause anxiety,deficient motor functions,and abnormal learning and memory in SD rats.

关 键 词：硝酸铈 神经行为 大鼠 亚慢性暴露 

分 类 号：R994.6[医药卫生—毒理学]