PM_(2.5)混悬液对3种癌细胞侵袭和迁移能力的影响  

Effects of PM_(2.5) on invasion and migration abilities in three types of cancer cells

作　　者：李柏茹 秦双建 蔡颖[2,3] 李闰冰[2,3] 关岚 肖芳[1] 曾明[1] 徐新云[3] LI Boru;QIN Shuangjian;CAI Ying;LI Runbing;GUAN Lan;XIAO Fang;ZENG Ming;XU Xinyun(Xiangya School of Public Health,Central South University,Changsha 410078,Hunan;School of Public Health,University of South China,Hengyang 421001,Hunan;Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,Guangdong,China)

机构地区：[1]中南大学湘雅公共卫生学院,湖南长沙410078 [2]南华大学公共卫生学院,湖南衡阳421001 [3]深圳市疾病预防控制中心,广东深圳518055

出　　处：《癌变．畸变．突变》2022年第2期124-128,133,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技研发项目(JCYJ20190807102205480)。

摘　　要：目的:探讨PM_(2.5)混悬液对人肺癌A549细胞、人肝癌HepG2细胞和人肾癌A498细胞迁移和侵袭能力的影响。方法:采用10、50μg/mL的PM_(2.5)混悬液分别对A549、HepG2和A498细胞染毒24 h,Transwell小室实验检测细胞迁移、侵袭能力。结果:迁移实验结果显示,阴性对照组、10和50μg/mL PM_(2.5)染毒组A549细胞穿膜细胞数分别为(356.33±30.92)、(476.33±28.50)和(563.33±74.00)个;HepG2细胞分别为(86.42±4.75)、(140.00±16.15)和(234.17±9.06)个;A498细胞分别为(42.33±6.43)、(80.00±6.08)和(174.67±25.01)个。侵袭实验结果显示,阴性对照组、10和50μg/mL PM_(2.5)染毒组A549细胞的穿膜细胞数分别为(188.33±9.45)、(313.00±7.94)和(404.00±57.66)个;HepG2细胞分别为(48.33±3.26)、(97.75±6.63)和(123.92±8.57)个;A498细胞分别为(5.67±5.03)、(21.33±5.13)和(40.67±6.81)个。在迁移实验和侵袭实验中,与阴性对照组相比,PM_(2.5)染毒组中的3种细胞穿膜细胞数均增加(P<0.05或0.01)。结论:PM_(2.5)可增强A549、HepG2和A498细胞的迁移和侵袭能力。OBJECTIVE:To investigate effects of PM_(2.5) on the migration and invasion abilities in three types of cancer cells:human lung(A549),human liver(HepG2)and human renal(A498)cancer cells.METHODS:The A549,HepG2 and A498 cells were exposed to PM_(2.5) suspension at 10 and 50μg/mL for 24 h.The transwell assay was performed to detect cell migration and invasion abilities.RESULTS:The results from the migration assay show that the number of transmembrane A549 cells in the negative control,10 and 50μg/mL PM_(2.5) exposure groups were(356.33±30.92),(476.33±28.50)and(563.33±74.00),respectively.Data for the HepG2 cells were(86.42±4.75),(140.00±16.15)and(234.17±9.06);and those for the A498 cells were(42.33±6.43),(80.00±6.08)and(174.67±25.01),respectively.The results from the invasion assay show that the number of transmembrane A549 cells in the three experimental groups were(188.33±9.45),(313.00±7.94)and(404.00±57.66),respectively.Data for the HepG2 cells were(48.33±3.26),(97.75±6.63)and(123.92±8.57);and those for the A498 cells were(5.67±5.03),(21.33±5.13)and(40.67±6.81),respectively.In the migration and invasion experiments,compared with the negative control group,the numbers of penetrating cells of the three types of cells in the PM_(2.5) exposure group were significantly increased(P<0.05).CONCLUSION:PM_(2.5) significantly enhanced the abilities of migration and invasion in A549,HepG2 and A498 cells.

关 键 词：大气细颗粒物 A549细胞 HEPG2细胞 A498细胞 侵袭 迁移 

分 类 号：R122.7[医药卫生—环境卫生学]