广东普通野生稻对水稻白叶枯病的抗性评价及分析  

Identification and Analysis of the Bacterial Blight Resistance of Common Wild Rice in Guangdong Province

作　　者：张静 范芝兰[1] 潘大建[1] 刘维[1] 江立群 吕树伟 孙炳蕊[1] 刘清[1] 毛兴学[1] 陈文丰[1] 李晨[1] ZHANG Jing;FAN Zhi-lan;PAN Da-jian;LIU Wei;JIANG Li-qun;LYU Shu-wei;SUN Bing-rui;LIU Qing;MAO Xing-xue;CHEN Wen-feng;LI Chen(Rice Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory 9 Guangzhou 510640)

机构地区：[1]广东省农业科学院水稻研究所/广东省水稻育种新技术重点实验室/广东省水稻工程实验室,广州510640

出　　处：《植物遗传资源学报》2022年第2期422-429,共8页Journal of Plant Genetic Resources

基　　金：广东省重点领域研发计划项目(2020B0202090003);广东省农作物种质资源专项(省长专项2018-2019)。

摘　　要：本研究通过抗性鉴定、分子标记和方差分析,评价与分析了广东普通野生稻对白叶枯病的抗性。结果表明广东普通野生稻对广东省典型的致病菌株Ⅳ型小种表现为抗或高抗,对PXO99小种的抗性反应差异较大,表现出从中感到抗病。用已知功能标记做鉴定,结果显示:20个普通野生稻居群中,3个居群能够扩增出Xa21抗病基因条带;4个居群能够扩增出Xa4抗病基因条带;5个居群能够扩增出Xa7、Xa27抗病基因条带;Xa23、Xa10、Xa26抗病基因条带分别能够在11、12和16个居群中被扩增出;19个居群能检测出Xa1的抗病基因条带,所有居群均不含xa5、xa13抗病基因。抗谱分析发现,HY2、DB2和GZ2 3个居群中的5份野生稻资源可能含有新的抗性基因。方差分析结果表明,对于Ⅳ型小种,高州普通野生稻的抗性水平显著高于化州、雷州和台山普通野生稻;而化州、台山普通野生稻的抗性水平显著低于陆丰普通野生稻。对于PXO99小种,广东不同县市间的抗性水平未呈现出显著性差异。本研究为进一步发掘和利用广东普通野生稻的优异抗白叶枯病基因提供了材料基础和理论依据。Bacterial blight(BB),as a destructive disease of rice worldwide,has seriously destabilized rice yield. The most effective and environmental-friendly method in disease control is to explore and utilize the BB resistance,whereas the known resistance genes to date are limited. Wild rice populations host tremendous resistance genes,and identification of resistance genes to bacterial blight is of great significance in rice genetic improvement. In this research,the resistance to BB in common wild rice population in Guangdong province was analyzed. Although different levels of resistance to BB strains PXO99 were observed,Guangdong wild rice populations presented resistance or high resistance to the typical pathogenic strain type IV race in Guangdong.According to the genotyping result with functional markers,three sub-populations were revealed containing resistant gene Xa21,four sub-populations containing Xa4,five sub-populations containing Xa7 and Xa27. Xa23,Xa10 and Xa26 were detected in 11,12 and 16 sub-populations,respectively. The resistance genes xa5 and xa13were not found,but 19 sub-populations contained Xa1 or its homologous gene. Resistance spectrum analysis showed that five common wild rice accessions from HY2,DB2 and GZ2 populations might contain new BB resistance genes. For BB strain race-IV,the results of variance analysis demonstrated that the BB resistance level of Gaozhou common wild rice was significantly higher than that of Huazhou,Leizhou and Taishan,while the resistance level of Huazhou and Taishan common wild rice was significantly lower than that of Lufeng. For PXO99 race,there was no significant difference in the resistance level among all wild materials. Collectively,this study provided a basis for further exploring and utilizing the excellent BB resistance gene resources of common wild rice in Guangdong province.

关 键 词：广东野生稻 白叶枯病 抗性鉴定 PCR分析 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]