Zn^(2+)交联海藻酸钠抑菌缓释微球制备及其抑菌效应  

Preparation and Antibacterial Effect of Zn;Crosslinked Alginate Slow-release Microspheres

作　　者：李玉妍 杨忠鑫 陈南春[1] 莫胜鹏 王秀丽[3] 解庆林[2] LI Yuyan;YANG Zhongxin;CHEN Nanchun;MO Shengpeng;WANG Xiuli;XIE Qinglin(School of Materials Science and Engineering,Guilin University of Technology,Guilin 541004,Guangxi,China;School of Environmental Science and Engineering,Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541006,Guangxi,China;School of Chemical and Biological Engineering,Guilin University of Technology,Guilin 541006,Guangxi,China)

机构地区：[1]桂林理工大学材料科学与工程学院,广西桂林541004 [2]桂林理工大学环境科学与工程学院,广西岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541006 [3]桂林理工大学化学与生物工程学院,广西桂林541006

出　　处：《材料导报》2022年第7期228-234,共7页Materials Reports

基　　金：国家自然科学基金(51564008,51978189);广西环境污染控制理论与技术重点实验室研究项目(1801K002)。

摘　　要：为提高二甲酸钾(KDF)在胃肠道内的利用率,本研究基于单因素实验采用响应面法优化设计数据,利用Zn^(2+)交联海藻酸钠(ALG)制备出KDF缓释抑菌微球,并对微球的化学结构、微观形貌及热性能进行系统表征,以及测定微球的缓释抑菌性能。结果表明,微球的最佳配方为:ALG浓度1.5%(质量分数,下同),ZnSO_(4)浓度7.0%,表面活性剂0.8 mL。优化后的微球在肠道内的释放作用时间可延长至6 h,释放机制遵循一级动力学模型和Ritger-Peppas模型,显著减缓了KDF的释放速率;微球浓度为96 mg/mL时,其对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抑菌率分别为88%、87%、67%,有效抑制了细菌的过度繁殖,是一种可在家畜饲养业广泛普及的新型抑菌剂。To improve the utilization of potassium diformate(KDF) in gastrointestinal tract, this study, which was based on the single factor experiment, optimized the design data by response surface methodology. The KDF microspheres were prepared by Zn^(2+) cross-linked sodium alginate(ALG). The obtained microspheres were systematically characterized by chemical structure, morphology and thermal properties, and the sustained-release antibacterial properties of the microspheres were tested. The results showed that the optimal formulation of microspheres was the concentration of ALG was 1.5%(mass fraction, the same below), the concentration of ZnSO_(4)was 7.0%, and the surfactant was 0.8 mL. In vitro slow-release properties of microspheres showed that the release time was prolonged to 6 h in the intestinal tract, and the release mechanism followed the first-order kinetic model and Ritger-Peppas model, which significantly slowed down the release rate of KDF. In addition, when the concentration of microspheres was 96 mg/mL, the bacteriostatic rates against Escherichia coli, Staphylococcus aureus and Bacillus subtilis were 88%, 87% and 67%, respectively. It effectively inhibits the overreproduction of bacteria and it was a potential new antimicrobial agent which could be widely used in livestock feeding.

关 键 词：二甲酸钾 响应面法 Zn^(2+)交联 微球 释放机制 抑菌率 

分 类 号：TB321[一般工业技术—材料科学与工程]