小蓟内生真菌Chaetomium Bostrychodes次级代谢产物及其生物活性研究  

Secondary Metabolites and Their Biological Activities of Chaetomium Bostrychodes fromCirsium Setosum

作　　者：吴迪 徐奥然 牟青林 鲜鹏杰 柳树直 黄泽雕 杨健[3] 王文静 杨小龙 Wu Di;Xu Aoran;Mu Qinglin;Xian Pengjie;Liu Shuzhi;Huang Zediao;Yang Jian;Wang Wenjing;Yang Xiaolong(The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province,School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan 430074,China;School of Pharmaceutical Sciences,Chongqing University,Chongqing 401331,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijng 100700,China)

机构地区：[1]中南民族大学药学院湖北省少数民族医学现代化工程技术研究中心,湖北武汉430074 [2]重庆大学药学院,重庆401331 [3]中国中医科学院国家中药资源中心道地药材国家重点实验室培育基地,北京100700

出　　处：《亚太传统医药》2022年第3期21-27,共7页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金(81872755、82073714、31900288);国家重点研发计划(2020YFC1712703);国家民委中青年英才培养计划(MZR20008);中南民族大学中央高校基本科研业务费优青培育项目(CZP 20008)。

摘　　要：目的:对一株小蓟内生旋丝毛壳菌(Chaetomium bostrychodes)次级代谢产物化学结构及生物活性进行研究。方法:利用柱层析、Sephadex-20、HPLC等方法对发酵产物进行分离纯化,经核磁共振波谱、高分辨质谱技术并结合参考文献确定化合物结构,采用二倍稀释法检测化合物的抗菌活性。结果:从上述发酵产物中分离得到9个单体化合物,分别为aurasperone A(1)、fonsecinone A(2)、granulolactone(3)、4-hydroxyphenylacetone(4)、latifolicinin C(5)、p-hydroxybenzoic acid ethylester(6)、p-hydroxybenzoic acid(7)、phallac acid A(8)、1,2,4-benzenetriol(9),以上化合物均为首次从该菌种中分离得到。对这些化合物的抗临床耐药细菌和植物病源真菌活性进行测试,结果显示均无显著抑制作用。Objective:The secondary metabolites and their biological activities of Chaetomium bostrychodes,an endophytic fungui isolated fromCirsium setosum,are investigated.Methods:The solid culture was separated and purified by column chromatography,Sephadex-20 and HPLC.The compounds were identified by NMR spectra,HRESIMS data and reference materials.The antibacterial and antifungal activity of the compounds was detected by two fold dilution method.Results:Nine compounds were isolated from the solid culture of C.bostrychodes.Their structures were identified as aurasperone A(1)、fonsecinone A(2)、granulolactone(3)、4-hydroxyphenylacetone(4)、latifolicinin C(5)、p-hydroxybenzoic acid ethylester(6)、p-hydroxybenz-oic acid(7)、phallac acids A(8)、1,2,4-benzenetriol(9)by the NMR spectra and HRESIMS data.All the above compounds were isolated from this strain for the first time and the biological activities of these compounds against clinical drug-resistant bacteria and plant pathogenic fungi were tested.However,none of them showed significant inhibitory activity.

关 键 词：小蓟 内生真菌 旋丝毛壳菌 次级代谢产物 生物活性 

分 类 号：R284[医药卫生—中药学]