DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia  

作　　者：Gang Xu Yi-Kun Shi Bin-Da Sun Lu Liu Guo-Ji E Shu He Jian-Yang Zhang Bao Liu Qiu Hu Jian Chen Yu-Qi Gao Er-Long Zhang 

机构地区：[1]Institute of Medicine and Equipment for High Altitude Region,College of High Altitude Military Medicine,Army Medical University,Chongqing 400038,China [2]Key Laboratory of Extreme Environmental Medicine,Ministry of Education of China,Chongqing 400038,China [3]Key Laboratory of High Altitude Medicine,People's Liberation Army,Chongqing 400038,China

出　　处：《Military Medical Research》2022年第1期1-11,共11页军事医学研究（英文版）

基　　金：supported by grants from the National Science and Technology Major Project(2014ZX09J14102-05B and 2018ZX09J18109)。

摘　　要：Background:Studies have revealed the protective effect of DL-3-n-butylphthalide(NBP)against diseases associated with ischemic hypoxia.However,the role of NBP in animals with hypobaric hypoxia has not been elucidated.This study investigated the effects of NBP on rodents with acute and chronic hypobaric hypoxia.Methods:Sprague-Dwaley rats and Kunming mice administered with NBP(0,60,120,and 240 mg/kg for rats and 0,90,180,and 360 mg/kg for mice)were placed in a hypobaric hypoxia chamber at 10,000 m and the survival percentages at 30 min were determined.Then,the time and distance to exhaustion of drug-treated rodents were evaluated during treadmill running and motor-driven wheel-track treadmill experiments,conducted at 5800 m for 3 days or 20 days,to evaluate changes in physical functions.The frequency of active escapes and duration of active escapes were also determined for rats in a shuttle-box experiment,conducted at 5800 m for 6 days or 27 days,to evaluate changes in learning and memory function.ATP levels were measured in the gastrocnemius muscle and malonaldehyde(MDA),superoxide dismutase(SOD),hydrogen peroxide(H_(2)O_(2)),glutathione peroxidase(GSH-Px),and lactate were detected in sera of rats,and routine blood tests were also performed.Results:Survival analysis at 10,000 m indicated NBP could improve hypoxia tolerance ability.The time and distance to exhaustion for mice(NBP,90 mg/kg)and time to exhaustion for rats(NBP,120 and 240 mg/kg)significantly increased under conditions of acute hypoxia compared with control group.NBP treatment also significantly increased the time to exhaustion for rats when exposed to chronic hypoxia.Moreover,240 mg/kg NBP significantly increased the frequency of active escapes under conditions of acute hypoxia.Furthermore,the levels of MDA and H_(2)O_(2) decreased but those of SOD and GSH-Px in the sera of rats increased under conditions of acute and chronic hypoxia.Additionally,ATP levels in the gastrocnemius muscle significantly increased,while lactate levels in sera significantly

关 键 词：DL-3-n-butylphthalide Hypobaric hypoxia Physical function Learning and memory function Oxidative stress Energy metabolism 

分 类 号：R339.5[医药卫生—人体生理学]