2型糖尿病视网膜病变大鼠模型建立  

Establishment of Type 2 Diabetic Retinopathy Rat Model

作　　者：朱晓燕 刘勤 白惠玲[3] 金涛[1] 张书[3] 张延英[2] 康万荣 ZHU Xiaoyan;LIU Qin;BAI Huiling;JIN Tao;ZHANG Shu;ZHANG Yanying;KANG Wanrong(The First Clinical Medical College of Gansu University of Traditional Chinese Medicine/Gansu Provincial Hospital,Lanzhou 730000,China;Research And Experiment Center,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Department of Ophthalmology,Gansu Provincial Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学第一临床医学院/甘肃省人民医院,兰州730000 [2]甘肃中医药大学科研实验中心,兰州730000 [3]甘肃省人民医院眼科,兰州730000

出　　处：《实验动物科学》2022年第1期28-33,共6页Laboratory Animal Science

基　　金：甘肃省卫生行业科研计划基金资助项目(GSWSKY-2019-40);甘肃省人民医院院内科研基金资助项目(20GSSY1-15)。

摘　　要：目的建立2型糖尿病视网膜病变大鼠模型。方法80只雄性SD大鼠随机分成两组:空白对照组(普通饲料)及T2DM组(高脂高糖饲料喂养4周联合30 mg/kg链脲佐菌素腹腔注射)。链脲佐菌素注射后观察大鼠一般情况、每周测量体质量、随机血糖、空腹血糖、空腹血清胰岛素等指标,计算并分析胰岛抵抗指数(HOMA-IR)。分别在血糖稳定后,第3、4、5及6个月各组取10只大鼠经麻醉和散瞳,行眼底荧光造影和病理形态检查,观察2型糖尿病大鼠视网膜改变。结果链脲佐菌素注射后第1周,T2DM组大鼠表现出多食、多饮、多尿及体质量减轻的糖尿病症状。随着病程的进展,T2DM组与空白对照组大鼠一般情况、体质量、随机血糖、HOMA-IR等各项指标均有明显改变,差异有统计学意义。T2DM大鼠3个月时,眼底荧光造影:眼底尚清晰、视网膜血管走行迂曲、可见微动脉瘤及少量点状强荧光;病理形态改变,如:各层细胞排列紊乱、神经节细胞层可见扩张的血管、内外核层细胞数量减少及排列稀疏。随着病程延长至4、5及6个月时,眼底荧光造影和病理形态改变也逐渐加重。结论成功构建T2DM视网膜病变大鼠模型,为今后该病发病机理、药效学等方面提供较理想的实验动物模型。Objective To establish a rat model of type 2 diabetic retinopathy.Method Eighty male SD rats were randomly divided into 2 groups.The blank control group(n=40)was fed ordinary diet and the type 2 diabetes group(n=40)was fed high-fat and high-sugar diet for 4 weeks with 30 mg/kg streptozotocin(STZ)intraperitoneal injection.General condition,body weight,random blood glucose,fasting blood glucose and fasting serum insulin(FINS)were measured weekly after STZ injection and HOMA-IR was calculated and analyzed.At the 3;,4;,5;and 6;months after blood glucose stabilization,10 rats from each group were anesthetized and pupil dilated and underwent fundus fluorescein angiography and pathological morphology examination to observe the retinal changes of type 2 diabetic rats.Result At the 1;week after STZ injection,rats in the type 2 diabetes(T2 DM)group showed diabetes symptoms of polydipsia,polydipsia,polyuria and weight loss.With the progress of disease course,the general condition,body weight,random blood glucose and HOMA-IR of rats in the T2 DM group and the blank control group were significantly changed,and the differences were statistically significant.Fundus fluorescein angiography was performed in the type 2 diabetic rats at 3 months:fundus was clear,retinal vessels were tortuous,microaneurysms and a small amount of spotty strong fluorescein were observed;pathological morphological changes:the cells in each layer were disordered,with dilated blood vessels in the ganglion cell layer,and the number of cells in the inner and outer nuclear layers was reduced and sparsely arranged.With the prolongation of the course of disease,fundus fluorescein angiography and pathological changes gradually became more serious at the 4,5 and 6 months.Conclusion A rat model of type 2 diabetic retinopathy was established,which provides an ideal laboratory animal model for pathogenesis and pharmacodynamics.

关 键 词：2型糖尿病 糖尿病视网膜病变 动物模型 眼底荧光造影 

分 类 号：Q95-33[生物学—动物学]