天降血栓通丸对ApoE^(-/-)小鼠动脉粥样硬化的干预作用及其机制研究  

Interventional effect of Tianjiang Xueshuantong pills on atherosclerosis in ApoE^(-/-) mice and its mechanism

作　　者：王居平 赵聪娜 张宝楠 杨维伦 施乐[1] WANG Juping;ZHAO Congna;ZHANG Baonan;YANG Weilun;SHI Le(Beichen Hospital of Traditional Chinese Medicine,Tianjin 300400,China)

机构地区：[1]天津市北辰区中医医院,天津300400

出　　处：《湖南中医杂志》2022年第2期164-168,共5页Hunan Journal of Traditional Chinese Medicine

基　　金：天津市北辰区科技计划项目(SHGY-2020038);天津市中医药管理局中医、中西医结合科研专项项目(2015147)。

摘　　要：目的:探讨天降血栓通丸对ApoE^(-/-)小鼠动脉粥样硬化的干预作用及其机制。方法:将30只动脉粥样硬化建模成功的ApoE^(-/-)小鼠随机分为模型组、阿托伐他汀组和天降血栓通丸组,每组各10只,分别给予0.9%氯化钠注射液、阿托伐他汀、天降血栓通丸药液灌胃8周。10只C57BL/6小鼠予普通饲料喂养,作为对照组,灌胃0.9%氯化钠注射液。干预结束后,检测血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、单核细胞趋化因子-1(MCP-1)、细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)水平,检测主动脉组织c-Jun氨基末端活化蛋白激酶(JNK)、丝裂原活化蛋白激酶(p38MAPK)mRNA的表达。结果:与模型组比较,阿托伐他汀组和天降血栓通丸组血清TG、TC、LDL-C、MCP-1、ICAM-1、VCAM-1水平及主动脉组织JNK mRNA、p38MAPK mRNA表达均降低,差异均有统计学意义(P<0.05);与对照组比较,模型组血清TG、TC、LDL-C、MCP-1、ICAM-1、VCAM-1水平及主动脉组织JNK mRNA、p38MAPK mRNA表达水平均升高,差异均有统计学意义(P<0.05)。结论:天降血栓通丸具有防治动脉粥样硬化的作用,其机制可能与抑制JNK/p38 MAPK信号,下调MCP-1、ICAM-1、VCAM-1表达,减轻炎症反应有关。Objective: To investigate the interventional effect of Tianjiang Xueshuantong pills on atherosclerosis in ApoE^(-/-) mice and its mechanism.Methods: A total of 30 ApoE^(-/-) mice with successful atherosclerosis modeling model, which were randomly divided into model group, atorvastatin group, and Tianjiang Xueshuantong pill group, with 10 mice in each group.The above three groups were given 0.9% sodium chloride injection, atorvastatin solution, and Tianjiang Xueshuantong pill solution, respectively, by gavage for 8 weeks.A total of 10 C57 BL/6 mice fed with normal diet were selected as control group and were given 0.9% sodium chloride injection by gavage.After intervention, the serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol, monocyte chemoattractant protein-1(MCP-1),intercellular adhesion molecule-1(ICAM-1),and vascular cell adhesion molecule-1(VCAM-1) were measured, as well as the mRNA expression of c-Jun N-terminal kinase(JNK) and p38 mitogen-activated protein kinase(p38 MAPK) in aortic tissue.Results: Compared with the model group, the atorvastatin group and the Tianjiang Xueshuantong pill group had significant reductions in the serum levels of TG,TC,LDL-C,MCP-1,ICAM-1,and VCAM-1 and the mRNA expression of JNK and p38 MAPK in aortic tissue(P<0.05).Compared with the control group, the model group had significant increases in the serum levels of TG,TC,LDL-C,MCP-1,ICAM-1,and VCAM-1 and the mRNA expression of JNK and p38 MAPK in aortic tissue(P<0.05).Conclusion: Tianjiang Xueshuantong pills have a certain effect in the prevention and treatment of atherosclerosis, possibly by inhibiting the JNK/p38 MAPK signal, downregulating the expression of MCP-1,ICAM-1,and VCAM-1,and alleviating inflammatory response.

关 键 词：动脉粥样硬化 天降血栓通丸 APOE基因敲除小鼠 实验研究 

分 类 号：R285.5[医药卫生—中药学]