等温扩增技术在疟原虫及巴贝虫检测中的应用  

Application of isothermal amplification technology in the detection of Plasmodium and babesia

作　　者：强焜 徐斌[1] 胡薇[1] 郑彬[1] QIANG Kun;XU Bin;HU Wei;ZHENG Bin(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),NHC Key Laboratory of Parasite and Vector Biology(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention),WHO Collaborating Center for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,School of Global Health,Chinese Center for Tropical Diseases,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心),国家卫生健康委员会寄生虫病原与媒介生物学重点实验室(中国疾病预防控制中心寄生虫病预防控制所),世界卫生组织热带病合作中心,科技部国家级热带病国际研究中心/上海交通大学医学院-国家热带病研究中心全球健康学院,上海200025

出　　处：《中国热带医学》2022年第1期84-88,共5页China Tropical Medicine

基　　金：国家科技重大专项(No.2018ZX10101002-002-003)。

摘　　要：近年来,等温扩增技术(isothermal amplification technology)凭借恒温条件下对核酸高效率扩增的优势在病原体检测领域展现出了巨大的潜力。疟疾与巴贝虫病作为两类严重危害人类健康的寄生虫病在临床表现与诊断方式上相似,存在误诊的风险。同时,这两类寄生虫病可通过血液传播。因此,高效、快速的检测技术对疟疾与巴贝虫病的诊断和保证血液制品安全有重要的意义。本文对现有针对疟原虫与巴贝虫的环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)、重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)、重组酶介导等温扩增技术(recombinase-aided amplification,RAA)以及依赖解旋酶等温扩增技术(helicase-dependent amplification,HDA)等四类等温扩增技术的原理、特点及检测方式进行综述并对其应用前景进行展望。In recent years,isothermal amplification technology has shown great potential in the field of pathogen detection by virtue of its advantages in high-efficiency amplification of nucleic acids under constant temperature conditions.Malaria and Babesia,as two types of parasitic diseases that seriously endanger human health,have similar clinical manifestations and diagnostic methods.Two types of parasitic diseases are at risk of misdiagnosis.At the same time,these two types of parasitic diseases can be spread through blood.Therefore,efficient and rapid detection technology is of great significance for the diagnosis of malaria and Babesiosis and ensuring the safety of blood products.In this paper,four types of isothermal amplification techniques,including loop-mediated isothermal amplification(LAMP),recombinase polymerase amplificatio(RPA),recombinase-aided amplification(RAA)and helicase-dependent amplification(HDA)for Plasmodium and Babesia,are reviewed in this article.The principle,characteristics and detection methods of technology are reviewed and their application prospects are prospected.

关 键 词：等温扩增技术 疟疾 巴贝虫病 

分 类 号：R531[医药卫生—内科学]