转基因耐除草剂大豆ZH10-6目标性状遗传稳定性分析  

Genetic Stability Analysis of Target Traits in Transgenic Herbicide Tolerant Soybean ZH10-6

作　　者：王成 王雅偲 李纳 姚俊津 徐石勇[1] 赵新[1] 兰青阔[1] 王永[1] WANG Cheng;WANG Ya-si;LI Na;YAO Jun-jin;XU Shi-yong;ZHAO Xin;LAN Qing-kuo;WANG Yong(Biotechnology Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China;College of Plant Protection,Heibei Agricultural University,Baoding 071001,China;College of Horticulture and Landscape Architectures,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津市农业科学院生物技术研究所,天津300381 [2]河北农业大学植物保护学院,河北保定071001 [3]天津农学院园艺园林学院,天津300392

出　　处：《大豆科学》2021年第6期767-773,共7页Soybean Science

基　　金：国家科技重大专项(2019ZX08013-002)。

摘　　要：为评估转G2-EPSPS和GAT基因耐除草剂大豆ZH10-6耐30%草甘膦水剂(孟山都,农达)目标性状在不同世代中的遗传稳定性,利用酶联免疫吸附测定法(ELISA),检测了转基因大豆ZH10-6不同世代(T_(2)-T_(4))、不同生育期以及不同器官中的目标蛋白含量。同时,通过喷施不同剂量的草甘膦,考察了3个世代转基因大豆对目标除草剂的耐受性。结果显示:供试转基因材料均能稳定检测出目标蛋白G2-EPSPS和GAT,且不同世代间的表达量基本一致。目标蛋白在叶片中表达量最高,在籽粒中最低。在喷施相同剂量的草甘膦条件下,3个世代的转基因大豆ZH10-6的株高、成苗率和受害率均与非转基因对照大豆存在显著差异(P<0.05),且对4倍中剂量的目标除草剂表现出了较强的耐受性。研究结果表明ZH10-6的目标性状能够在后代中稳定遗传。In order to evaluate the genetic stability of the target traits of 30%glyphosate water(Monsanto)tolerance in transgenic herbicide tolerant soybean at different generations,we used transgenic soybean ZH10-6 with G2-EPSPS and GAT genes as materials to detect the target protein content in different generations(T_(2)-T_(4)),growth stages and organs of transgenic soybean by enzyme-linked immunosorbent assay(ELISA).At the same time,we investigated the tolerance of three generations of transgenic soybean to the target herbicide by spraying different doses of glyphosate.The results showed that,the target proteins G2-EPSPS and GAT could be detected stably in all transgenic materials,and the expression levels were basically the same among different generations.The expression of target protein was the highest in leaves and the lowest in seeds.Under the condition of spraying the same dose of glyphosate,the plant height,seedling rate and damage rate of transgenic soybean ZH10-6 in three generations were significantly different to those of non-transgenic control soybean(P<0.05),and showed strong tolerance to 4 times medium dose of target herbicide.The results showed that ZH10-6 could be stably inherited in the offspring,which provided data support for the biosafety evaluation of transgenic herbicide tolerant soybean.

关 键 词：大豆 转基因 耐除草剂 目标性状 遗传稳定性 

分 类 号：S565.1[农业科学—作物学]