山东济宁大豆病毒病的病原鉴定研究  

ldentification of the Viral Pathogens Infecting Soybean in Jining,Shandong Province

作　　者：赵小慧 吴茜 陈正强 刘冲[1] 邢锦城[1] 陶小荣[2] ZHAO Xiao-hui;WU Qian;CHEN Zheng-qiang;LIU Chong;XING Jin-cheng;TAO Xiao-rong(Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng 224002,China;Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224002 [2]南京农业大学植物保护学院,江苏南京210095

出　　处：《大豆科学》2021年第6期829-834,共6页Soybean Science

基　　金：国家自然科学基金(31901856);2018年度沿海盐碱地农业科学观测实验站开放课题(YHS201801)。

摘　　要：大豆病毒病是世界各地大豆产区普遍发生的病害之一,为明确山东济宁试验田大豆病毒病种类和发生趋势,本研究利用斑点-酶联免疫吸附试验(Dot-blot ELISA)针对SMV病毒,采用反转录PCR(RT-PCR)针对14种病毒,针对14种病毒,对采自该地区的37份病样进行鉴定。结果发现:22份样品检出大豆花叶病毒(Soybean Mosaic Virus,SMV),检出率为51.3%;18份样品检出菜豆普通花叶病毒(Bean Common Mosaic Virus,BCMV),检出率为32.4%;在检测出病毒的23份样品中,6份样品是SMV和BCMV复合侵染,复合侵染率为24.3%。本研究首次报道了在山东济宁的大豆种植区发现BCMV侵染,研究结果能够为当地大豆病毒病防控和抗病育种方向提供一定的理论支持。Soybean virus disease is a devastating disease occurring in soybean production areas all over the world.In order to clarify the type and prevalence of soybean virus disease in the Jining test field in Shandong,we used the dot enzyme linked immunosorbent assay(Dot-blot ELISA)to identify SMV,and reverse transcription PCR(RT-PCR)to identify 14 kinds of viruses in 37 samples collected from this area.It was found that among all samples,22 samples were detected as soybean mosaic virus(SMV),with a detection rate of 51.3%,18 samples as bean common mosaic virus(BCMV),with a detection rate of 32.4%.Among the 23 samples with virus detected,six were compound infections of SMV and BCMV with compound infection rate of 24.3%.This is the first time that BCMV infection has been detected in soybean planting areas in Jining,Shandong Province,providing a direction for local soybean virus disease prevention,control and disease-resistant breeding.

关 键 词：大豆病毒病 大豆花叶病毒 菜豆普通花叶病毒 复合侵染 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]