桂皮醛抗念珠菌的敏感性及对细胞壁的影响  

Sensitivity of cinnamaldehyde to Candida and its effect on cell wall

作　　者：邓洁华[1,2,3] 王佳 祁晓明[5] 王刚生 DENG Jiehua;WANG Jia;QI Xiaoming;WANG Gangsheng(Department of Dermatology and Venereology,Second Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China;Hebei Clinical Research Center for Dermatology and Venereology,Shijiazhuang,Hebei 050000,China;Construction Unit of Sub-center of National Clinical Medical Research Center for Skin and Immune Diseases,Shijiazhuang,Hebei 050000,China;Scientific Research Department,Second Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China;Electron Microscope Room Department,Second Hospital of Hebei Medical University,Shijia-zhuang,Hebei 050000,China;Translational Medicine Center,Second Hospital of Hebei Medical University,Shijia-zhuang,Hebei 050000,China)

机构地区：[1]河北医科大学第二医院皮肤性病科,河北石家庄050000 [2]河北省皮肤病与性病临床医学研究中心,河北石家庄050000 [3]国家皮肤与免疫疾病临床医学研究中心分中心建设单位,河北石家庄050000 [4]河北医科大学第二医院科研处,河北石家庄050000 [5]河北医科大学第二医院电镜室,河北石家庄050000 [6]河北医科大学第二医院转化医学中心,河北石家庄050000

出　　处：《河北工业科技》2022年第2期123-128,共6页Hebei Journal of Industrial Science and Technology

基　　金：河北省自然科学基金(H2013206316)。

摘　　要：为了研发靶向抗真菌药物,研究桂皮醛对念珠菌抗菌活性及对白念珠菌细胞壁的影响。参照CLSI的M27-A3方案,采用微量液基稀释法,测定桂皮醛对念珠菌(白念珠菌、热带念珠菌、克柔氏念珠菌)的抗菌作用。将白念珠菌的菌悬液(1×10^(8)cfu/mL)加入到RPMI 1640液体培养基内,置于37℃孵育48 h后,离心、制片,在透射电镜下观察细胞结构,以卡泊芬净、氟康唑作对照。结果表明桂皮醛、卡泊芬净、氟康唑对念珠菌的MIC分别为白念珠菌3.91,15.62,31.25 mg/L,热带念珠菌3.91,15.62,31.25 mg/L,克柔氏念珠菌3.91,31.25,>250 mg/L)。电镜显示,0.1%(质量分数)的桂皮醛只作用于白念珠菌细胞壁,致细胞壁外层溶解脱落,细胞核及细胞器溶解消失,但细胞膜仍然完整;0.1%(质量分数)的卡泊芬净致白念珠菌细胞壁完全溶解脱落变薄,同时细胞膜部分受损;0.1%(质量分数)的氟康唑致白念珠菌细胞膜溶解脱落,但细胞壁较完整。桂皮醛、卡泊芬净对念珠菌(白念珠菌、热带念珠菌、克柔氏念珠菌)均有较强的抗菌活性,氟康唑对白念珠菌、热带念珠菌也有较强的抗菌活性,但对克柔氏念珠菌显示耐药。桂皮醛的抗菌活性优于卡泊芬净,卡泊芬净优于氟康唑。桂皮醛作用于白念珠菌细胞壁而不影响细胞膜,卡泊芬净作用于白念珠菌细胞壁但对细胞膜也有一定影响,氟康唑作用于白念珠菌细胞膜,而不影响细胞壁。因此,桂皮醛有望成为一种靶向治疗念珠菌感染的理想药物。In order to develop targeted antifungal drugs,the antifungal activity of cinnamaldehyde on Candida and its effect on the cell wall of Candida albicans were investigated.According to M27-A3 procedure of CLSI,the antibacterial effect of cinnamaldehyde on Candida(Candida albicans,Candida tropicalis,and Candida krusei)was determined by the micro-liquid dilution method.The Candida albicans suspension(1×10^(8) cfu/mL)was added to RPMI 1640 liquid medium,incubated at 37℃for 48 h,centrifuged to prepare sections,and the cell structure was observed by transmission electron microscope,and Caspofungin and fluconazole were used as controls.The results show that the MIC of cinnamaldehyde,caspofungin and fluconazole against Candida are as follows:Candida albicans 3.91,15.62,31.25 mg/L,Candida tropicalis 3.91,15.62,31.25 mg/L,Candida krusei 3.91,31.25,>250 mg/L.Electron microscope shows that 0.1%cinnamaldehyde only acts on the cell wall of Candida albicans,causing the outer layer of the cell wall to dissolve and fall off,the nucleus and organelles to dissolve and disappear,but the cell membrane is still intact;0.1%caspofungin causes the cell wall of Candida albicans to dissolve,fall off and thin,and the cell membrane is partially damaged.0.1%fluconazole dissolves the cell membrane of Candida albicans,but the cell wall is relatively intact.Cinnamaldehyde and caspofungin have strong antibacterial activity against Candida(Candida albicans,Candida tropicalis,and Candida krusei),and fluconazole has strong antibacterial activity against Candida albicans and Candida tropicalis,but it shows resistance to Candida krusei.The antibacterial activity of cinnamaldehyde is better than caspofungin,and caspofungin is better than fluconazole.Cinnamaldehyde acts on the cell wall of Candida albicans without affecting the cell membrane.Caspofungin acts on the cell wall of Candida albicans but also has a certain effect on the cell membrane.Fluconazole acts on the cell membrane of Candida albicans without affecting the cell wall.Cinnamaldehyde is e

关 键 词：中医学与中药学其他学科 桂皮醛 卡泊芬净 白念珠菌 氟康唑 抗菌活性 细胞壁 细胞膜 电镜 

分 类 号：R285.5[医药卫生—中药学]