气相色谱法检测食用油中的抗氧化剂叔丁基对羟基茴香醚、2,6-二叔丁基对甲基苯酚和叔丁基对苯二酚  

Detection of antioxidants,butylated hydroxyanisole,2,6-dit-butyl-p-methyl phenol and tertiary butylhydroquinone in edible oil by gas chromatography

作　　者：彭瑾 王红 周茜 刘畅 宁程茜 尧聪 PENG Jin;WANG Hong;ZHOU Qian;LIU Chang;NING Cheng-xi;YAO Cong Hubei(Product Quality Supervision and Inspection Research Institute,Wuhan,Hubei 430061,China)

机构地区：[1]湖北省产品质量监督检验研究院,湖北武汉430061

出　　处：《中国卫生检验杂志》2022年第4期399-401,405,共4页Chinese Journal of Health Laboratory Technology

基　　金：湖北省质量技术监督局科技计划项目(Hbj-kj201-810)

摘　　要：目的建立同时测定食用油中叔丁基对羟基茴香醚(BHA)、2,6-二叔丁基对甲基苯酚(BHT)、叔丁基对苯二酚(TBHQ)的气相色谱法。方法食用油样品经乙腈饱和的正己烷溶解后,采用正己烷饱和的乙腈提取,合并3次提取液,氮吹后用乙酸乙酯∶环己烷(1∶1,V/V)定容至2 ml供气相色谱仪分析用。结果3种抗氧化剂的加标回收率在89.18%~109.11%,相对标准偏差为0.95%~2.83%。BHA和BHT的最低检出限均为0.2μg/ml,TBHQ的最低检出限为0.4μg/ml,在10μg/ml~500μg/ml的线性范围内,线性相关系数均>0.999。混合标准物质基质工作曲线在0.02 g/kg~0.2 g/kg浓度范围内的线性相关系数均>0.99。结论该方法简单、快速、稳定、可靠,适用于食用油中BHA、BHT和TBHQ的快速定量检测。Objective To develop a method of determining 3 kinds of antioxidants[butylated hydroxyanisole(BHA),2,6-dit-butyl-p-methyl phenol(BHT)and tertiary butylhydroquinone(TBHQ)]in edible oil by gas chromatography simultaneously.Methods The edible oil sample was dissolved by n-hexane saturated with acetonitrile and extracted by n-hexane-saturated acetonitrile.The extract was combined for three times.After nitrogen blowing,ethyl acetate∶cyclohexane(1∶1,V/V)was used for gas chromatograph analysis.Results The recoveries of the three antioxidants ranged from 89.18%to109.11%,with the relative standard deviations(RSDs)of 0.95%-2.83%.The lowest limit of detection for BHA and BHT was 0.2μg/ml,and the lowest limit of detection for TBHQ was 0.4μg/ml.The linear correlation coefficients were greater than0.999 in the linear range of 10μg/ml-500μg/ml.The linear correlation coefficients of the working curves of the mixed standard materials were greater than 0.99 in the range of 0.02 g/kg-0.2 g/kg.Conclusion The method is simple,rapid,stable and reliable.It is suitable for rapid quantitative determination of BHA,BHT and TBHQ in edible oil.

关 键 词：气相色谱法 抗氧化剂 叔丁基对羟基茴香醚 2 6-二叔丁基对甲基苯酚 叔丁基对苯二酚 

分 类 号：O657[理学—分析化学]