INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages  

作　　者：Aya M.Fadl Elshymaa A.Abdelnaby Hossam R.El-Sherbiny 

机构地区：[1]Theriogenology Department,Faculty of Veterinary Medicine,Cairo University,Egypt

出　　处：《Asian pacific Journal of Reproduction》2022年第2期100-104,共5页亚太生殖杂志（英文版）

摘　　要：Objective:To investigate the effect of INRA82 extender on ram semen quality preserved in cooled and frozen conditions in comparison with Tris-based extenders and to select the suitable extender for ram semen preservation.Methods:Semen samples were collected and pooled from Awassi rams(n=5)and divided into three aliquots.Each aliquot was diluted with one of the following extenders:(1)Tris-citrate glucose,(2)Triscitrate fructose,and (3)INRA-82.For liquid storage,diluted samples were kept at 5℃ for 72 h.Progressive motility was measured at 0,24,48 and 72 h after cooling.Besides,viability,morphology,membrane,acrosome and DNA integrities were evaluated at 24 h post-cooling.For cryopreservation,diluted samples were cooled,equilibrated,loaded and frozen in liquid nitrogen.Thawed samples were evaluated in the same manner as cooled conditions.Results:Seminal characteristics were improved after cooling and thawing in samples diluted with INRA-82 compared to those diluted with Tris-citrate glucose or Tris-citrate fructose(P<0.05).Conclusions:Dilution of ram semen with INRA-82 improves semen parameters.Hence,INRA-82,as a stimulating diluent,can be successfully used to reserve viability and sustainability of cooled and cryopreserved ram semen.

关 键 词：Cryopreservation Dilution INRA-82 RAM Tris-citrate glucose Tris-citrate fructose 

分 类 号：X70[环境科学与工程—环境工程]