周期性机械牵拉通过上调Runx2表达促进MC3T3-E1细胞迁移  

Cyclic Stretch Promotes Migration of MC3T3-E1 Cells via Increasing Runx2 Expression

作　　者：闫旭 李子通 刘继婷 齐颖新[2] 梅炯[1] YAN Xu;LI Zitong;LIU Jiting;QI Yingxin;MEI Jiong(Department of Orthopedics,Shanghai Sixth People’s Hospital,Shanghai 200233,China;Institute of Mechanobiology and Medical Engineering,School of Life Science and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海市第六人民医院骨科,上海200233 [2]上海交通大学力学生物学研究所,上海200240

出　　处：《医用生物力学》2022年第1期162-168,共7页Journal of Medical Biomechanics

基　　金：上海交通大学医工交叉研究基金(YG2017MS16)。

摘　　要：目的探究周期性牵拉对小鼠胚胎成骨细胞MC3T3-E1迁移功能的影响及其相关机制。方法使用应变加载系统对体外培养的MC3T3-E1细胞施加15%幅度的牵拉,模拟细胞体内受力情况。使用划痕愈合实验检测MC3T3-E1细胞的迁移功能,使用蛋白免疫印迹法检测Runx2表达情况,使用RNA干扰技术特异性降低Runx2表达量。结果幅度15%、频率1.25 Hz、持续24 h的周期性机械牵拉可以促进MC3T3-E1细胞的迁移,升高细胞内Runx2表达水平。在静态培养条件下,干扰Runx2能抑制MC3T3-E1细胞的迁移。干扰MC3T3-E1细胞中Runx2表达可以部分降低机械牵拉对细胞迁移的促进效应。结论周期性牵拉可以促进MC3T3-E1细胞的迁移,在此过程中Runx2可能发挥重要作用。研究结果为寻找促进骨折愈合的创新临床治疗方法提供实验依据。Objective To investigate the effects of cyclic stretch on migration of MC3T3-E1 cells and its related mechanism.Methods The strain loading system was used to stretch MC3T3-E1 cells cultured in vitro with 15%amplitude,to simulate the mechanical condition in vivo.The wound healing assay was used to detect the migration of MC3T3-E1 cells.Western blotting was used to test Runx2 expression.RNA interfering was used to decrease Runx2 expression.Results Cyclic mechanical stretch with 15%amplitude,1.25 Hz frequency and lasting for 24 hours could promote the migration of MC3T3-E1 cells and increase the expression level of Runx2.Runx2 interference inhibited the migration of MC3T3-E1 cells in static culture condition.Interference with Runx2 expression in MC3T3-E1 cells could partially reduce the positive effect of cyclic mechanical stretch on cell migration.Conclusions Cyclic stretch can promote the migration of MC3T3-E1 cells,and Runx2 may play an important role in this process.This study provides experimental basis for finding innovative clinical treatment method to promote fracture healing.

关 键 词：周期性机械牵拉 细胞迁移 成骨细胞 骨折愈合 

分 类 号：R318.01[医药卫生—生物医学工程]