用于谷胱甘肽检测的新型罗丹明衍生物荧光探针  

Novel Rhodamine derivative fluorescent probe for detection of glutathione

作　　者：林一凡 王鹏[1] 郝振铭 莫善雁 曾程初[1] 胡利明[1] LIN Yifan;WANG Peng;HAO Zhenming;MO Shanyan;ZENG Chengchu;HU Liming(College of Life Science and Chemistry,Beijing Key Laboratory of Environmental and Oncology,Faculty of Environment and Life,Beijing University of Technology,Beijing 100124)

机构地区：[1]北京工业大学环境与生命学部,北京市环境与病毒肿瘤学重点实验室,北京100124

出　　处：《分析试验室》2022年第3期296-301,共6页Chinese Journal of Analysis Laboratory

基　　金：北京市自然科学基金与北京市教育委员会科技计划重点项目(KZ202110005003);北京市环境与病毒肿瘤学重点实验室经费(049000546320521)资助。

摘　　要：合成了一种反应型近红外荧光探针N-Rh-GSH,该探针以罗丹明衍生物为荧光母体,通过与谷胱甘肽(GSH)作用触发螺环的开关来实现信号的响应,其开环释放的荧光产物具有760 nm的近红外发射波长。细胞实验表明,该探针可实现对活细胞中GSH的成像。It is of great significance to design a fluorescent probe to monitor glutathione(GSH)in living cells for disease researches.Herein,we reported a reactive near-infrared(NIR)fluorescent probe N-Rh-GSH for the detection of GSH.The Rhodamine derivative was used as the fluorescent matrix for the probe,and the response signal was realized through action of glutathione(GSH)to trigger the switch of the screw.The probe N-Rh-GSH showed favourable selectivity and fast response towards GSH,and the fluorescent matrix released by the open loop exhibited extremely strong fluorescence signals at 760 nm.The living cell imaging assays demonstrated that the probe N-Rh-GSH can successfully track GSH.

关 键 词：谷胱甘肽 近红外 荧光探针 反应型 

分 类 号：O657.3[理学—分析化学]