iucA基因缺失对尿路致病性大肠埃希菌增殖、黏附、侵袭和定植能力的影响  

The effect of iucA gene deletion on the proliferation,adhesion,invasion and colonization ability of Uropathogenic E.coli

作　　者：邓聪[1] 康嘉乐[1] 林梅双[1] 林艳芬[1] Deng Cong;Kang Jiale;Lin Meishuang;Lin Yanfen(Dept Of Clinical laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260)

机构地区：[1]广州医科大学附属第二医院检验科,广州510260

出　　处：《安徽医科大学学报》2022年第2期177-181,共5页Acta Universitatis Medicinalis Anhui

基　　金：广东省医学科研基金(编号:A2019472)。

摘　　要：目的为探究铁摄取相关基因iucA在尿路致病性大肠埃希菌(UPEC)致病中的作用,利用UPEC模式菌株CFT073,通过λRed同源重组方法构建iucA基因缺失株ΔiucA,同时构建回补株C-iucA。方法测定A600绘制CFT073、ΔiucA和C-iucA菌株在LB液体培养基和无菌尿液中的增殖曲线。比较CFT073、ΔiucA和C-iucA菌株对人膀胱癌上皮细胞株5637体外黏附和侵袭能力。构建小鼠尿路感染模型,检测CFT073、ΔiucA和C-iucA菌株在膀胱的定植能力。结果CFT073、ΔiucA和C-iucA菌株在LB液体培养基中增殖速率相似(P=0.153)。ΔiucA在无菌尿液中的增殖速率低于CFT073(P=0.001),C-iucA的增殖速率较ΔiucA有所上升(P=0.005)。ΔiucA对5637细胞的黏附和侵袭能力均低于CFT073(P=0.007、0.002)。C-iucA的黏附和侵袭能力较ΔiucA有所上升(P=0.046、0.037)。ΔiucA在小鼠膀胱的定植能力低于CFT073和C-iucA(P=0.002、0.017)。结论iucA基因可能通过促进UPEC增殖、黏附和侵袭以及在靶器官的定植能力在UPEC致病中发挥作用。Objective To investigate the role of iron uptake-related gene iucA in the pathogenesis of Uropathogenic E.coli(UPEC),UPEC CFT073 strain was used to construct the iucA gene deletion strainΔiucA byλRed homologous recombination technology,and the complemented strain C-iucA was also constructed.Methods The proliferation rates of CFT073,ΔiucA and C-iucA in Luria-Bertani liquid medium and sterile urine were determined by measuring the absorbance at 600 nm.Cultured 5637 human bladder cancer epithelial cells confluent monolayers were incubated with CFT073,ΔiucA and C-iucA for adhesion and invasion ability assay.C57BL/6 mouse urinary tract infection model was constructed to assess colonization ability of CFT073,ΔiucA and C-iucA in murine bladder tissues.Results Data showed that CFT073,ΔiucA and C-iucA displayed the similar proliferation curves in Luria-Bertani liquid medium(P=0.153).The proliferation rate of△iucA in sterile urine was lower than that of CFT073(P=0.001),while the proliferation rate of C-iucA in sterile urine was significantly higher than that ofΔiucA(P=0.005).△iucA demonstrated much lower adhesion and invasion ability compared with CFT073(P=0.007,0.002),and C-iucA showed significantly higher adhesion and invasion ability than△iucA(P=0.046,0.037).The colonization ability of△iucA in murine bladder tissues was much lower than that of CFT073 and C-iucA(P=0.002,0.017).Conclusion The results indicate iucA gene may contribute to the proliferation,adhesion,invasion and colonization ability of UPEC.

关 键 词：iucA基因 大肠埃希菌 尿路感染 铁载体 基因缺失 

分 类 号：R378.21[医药卫生—病原生物学]