LSS基因功能缺失对雄性小鼠生殖的影响  

The effects of knockout of LSS on female mice fertility

作　　者：孙晓梅 李名聪 李涛[3] 魏翔 葛若木 张军[1] 李昱昊 周青[1] 汪渊[1] 张胜权[1] 张素梅[1] Sun Xiaomei;Li Mingcong;Li Tao;Wei Xiang;Ge Ruomu;Zhang Jun;Li Yuhao;Zhou Qing;Wang Yuan;Zhang Shengquan;Zhang Sumei(Dept of Biochemistry and Laboratory of Molecular Biology of Anhui Medical University,Key Laboratory of Gene Utilization for Severe Disease of Anhui Province,Hefei 230032;Dept of Laboratory Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230032;Dept of Pathology,Hefei Second People's Hospital,Hefei 230011;Dept of Hyperbaric Oxygen,Hefei Second People's Hospital,Hefei 230032)

机构地区：[1]安徽医科大学分子生物学实验室,生物化学与分子生物学教研室,安徽省省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032 [2]合肥市第二人民医院病理科,合肥230011 [3]安徽医科大学第一附属医院检验科,合肥230032 [4]合肥市第二人民医院高压氧科,合肥230011

出　　处：《安徽医科大学学报》2022年第2期208-212,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学研究项目(编号:KJ2020A0143)。

摘　　要：目的研究羊毛固醇合成酶(LSS)基因功能缺失对小鼠生殖的影响。方法取8周龄的LSS^(+/+)(WT)型和LSS^(+/-)(KO)型鼠雌雄共30只鼠,分别按♂WT×♀WT、♂WT×♀KO、♂KO×♀WT、♂KO×♀KO分组进行1:1合笼,时长4个月,统计每胎生仔个数及平均产仔周期;取WT型和KO型小鼠的血清,进行性激素检测;用TUNEL染色观察睾丸中生精细胞的凋亡情况;用Western blot检测睾丸组织中Tubulin、LSS和凋亡相关蛋白(GPX4/Bax/Bcl-2)的表达。结果配繁结果显示♂WT×♀WT与♂KO×♀KO相比,每胎平均生仔个数差异有统计学意义,其余组别均无差异,产仔周期4组的差异均无统计学意义;WT型和KO型小鼠性激素检测结果相比,差异也无统计学意义;TUNEL染色结果可以观察到,基因敲除鼠的睾丸组织中生精细胞凋亡增多;Western blot结果中,与WT型相比,KO型小鼠的凋亡相关蛋白(GPX4/Bax/Bcl-2)表达量均无差异。结论LSS基因功能缺失会导致小鼠生精细胞凋亡增加,其可能影响小鼠的生育能力。Objective To investigate the effect of lansterol synthase(LSS)gene knockout on reproductive function in male mice.Methods 30 male and female 8-week-old LSS+/+(WT)and LSS+/-(KO)mice were selected,and respectively according to♂WT×♀WT,♂WT×♀KO,♂KO×♀WT,♂KO×♀KO carries out 1∶1 cages for four months,and the number of litters per litter and reproductive cycle were counted.The serum of LSS^(+/+)(WT)and LSS^(+/-)(KO)mice was taken for sex hormone testing.The apoptosis of spermatogenic cells in the testis was observed by TUNEL staining.Western blot was used to detect the expression of Tubulin,LSS and apoptosis-related protein(GPX4/Bax/Bcl-2)in testis tissue.Results The breeding results showed that there was a statistically difference in the number of litters per litter between♂WT×♀WT and♂KO×♀KO,and there was no difference in the other groups.There was no statistically difference in the four groups in the reproductive cycle.There was no statistically difference between the sex hormone test results of WT and KO mice.TUNEL staining results showed that there was an increase in spermatogenic cell apoptosis in the testis tissue of the knockout mice.In the results of Western blot,there was no difference in the expression of apoptosis-related proteins(GPX4/Bax/Bcl-2)in KO mice compared with WT mice.Conclusion Loss of LSS gene function can lead to increased apoptosis of mouse spermatogenic cells,which may affect the fertility of mice.

关 键 词：羊毛固醇合成酶 基因敲除小鼠 凋亡 性激素 生殖 

分 类 号：R339.2[医药卫生—人体生理学]