子宫腺肌病在位/异位内膜TRPV 1、OTR的表达与痛经的相关性研究  

Expression of TRPV 1 and OTR in eutopic/ectopic endometrium and their correlation with dysmenorrhea in adenomyosis

作　　者：陈果 朱雨婷 姚慧[1] 张炜煜 周曙光 蒋晓敏[1,2] Chen Guo;Zhu Yuting;Yao Hui;Zhang Weiyu;Zhou Shuguang;Jiang Xiaomin(Dept of Gynecology, Maternal and Child Health HospitalAffiliated to Anhui Medical University, Hefei 230001;Dept of Gynecology, Anhui Province Maternity and Child Health Hospital, Hefei 230032)

机构地区：[1]安徽医科大学附属妇幼保健院,合肥230001 [2]安徽省妇幼保健院妇科,合肥230032

出　　处：《安徽医科大学学报》2022年第2期273-278,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究与开发计划项目(编号:201904a07020008);安徽医科大学校科研基金(编号:2019xkj173)。

摘　　要：目的探讨瞬时受体电位香草酸亚型1(TRPV 1)、催产素受体(OTR)在子宫腺肌病痛经发生机制中的作用。方法子宫腺肌病手术患者60例,分别取异位内膜(EC)和同源在位内膜(EU),并以无子宫内膜异位症患者子宫内膜(CE)为对照。采用免疫组化SP法检测TRPV 1、OTR在组织中的定位,Western blot检测TRPV 1、OTR的蛋白表达水平。通过视觉模拟评分法(VAS)对痛经程度评分,并分析其与上述因子的相关性。结果TRPV 1、OTR在EC组中表达均高于EU、CE组(P<0.05),而EU组与CE组之间差异无统计学意义。EC组中TRPV 1、OTR在重度痛经组中表达均高于轻度及中度组(P<0.05),而在轻度痛经组与中度痛经组之间差异无统计学意义。TRPV 1、OTR的表达与痛经严重程度呈正相关(rs=0.280,P<0.05;rs=0.318,P<0.05);TRPV 1与OTR的表达呈正相关(rs=0.287,P<0.05)。结论TRPV 1和OTR可能在子宫腺肌病患者痛经的致病机制中发挥协同作用,是痛经严重程度的重要预测因子,是潜在的治疗靶点。Objective To investigate the roles of transient receptor potentia1 vanilloid 1(TRPV 1)and oxytocin receptor(OTR)in the mechanism of dysmenorrhea in adenomyosis.Methods The ectopic endometrium(EC)and the homologous eutopic endometrium(EU)were selected from 60 patients with adenomyosis surgery,and the control endometrium(CE)of patients without endometriosis was used as control.Streptavidin-Peroxidase immunohistochemistry staining(SP)was used to detect the localization of TRPV 1 and OTR in the tissues,and the expression of TRPV 1 and OTR was detected by Western blot.The degree of dysmenorrhea was scored by visual analogue scale/score(VAS),and its correlation with the TRPV 1 and OTR was analyzed.Results The expression of TRPV 1 and OTR in EC was higher than that in EU and CE(P<0.05),but there was no statistically significant difference between EU and CE.In EC,the expression of TRPV 1 and OTR in the severe dysmenorrhea group was higher than that in the mild and moderate groups(P<0.05),but there was no statistically significant difference between the mild dysmenorrhea group and the moderate group.The expression of TRPV 1 and OTR was positively correlated with the severity of dysmenorrhea(r_(s)=0.280,P<0.05;r_(s)=0.318,P<0.05);the expression of TRPV 1 and OTR was positively correlated(r_(s)=0.287,P<0.05).Conclusion TRPV 1 and OTR may play a synergistic role in the pathogenesis of dysmenorrhea in patients with adenomyosis,which are important predictors of dysmenorrhea severity and potential therapeutic targets.

关 键 词：子宫腺肌病 瞬时受体电位香草酸亚型1 催产素受体 痛经 

分 类 号：R711.71[医药卫生—妇产科学]