锝[^(99)Tc]亚甲基二膦酸盐注射液治疗类风湿关节炎患者前后的肠道菌群变化  

Changes of gut microbiota before and after treatment of technetium[^(99)Tc]methylenediphosphonate injection in rheumatoid arthritis

作　　者：陈昌明 黄钊炜 曾苹 姚血明 刘灿 王莹 马武开 Chen Changming;Huang Zhaowei;Zeng Ping;Yao Xueming;Liu Can;Wang Ying;Ma Wukai(Dept of Rheumatology and Immunology, The Second Affiliated Hospital of Guizhou Universityof Traditional Chinese Medicine,Guiyang 550001)

机构地区：[1]贵州中医药大学第二附属医院风湿免疫科,贵阳550001

出　　处：《安徽医科大学学报》2022年第2期310-315,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81760907);贵州省科技计划项目(编号:黔科合支撑[2020]4Y155号、黔科合平台人才[2016]5650、黔科合平台人才[2020]2202号)。

摘　　要：目的探讨锝[^(99)Tc]亚甲基二膦酸盐注射液(^(99)Tc-MDP)治疗类风湿关节炎(RA)患者前后的肠道菌群变化。方法采集RA患者在应用^(99)Tc-MDP治疗前和治疗后的新鲜粪便样本各32例,提取粪便基因组DNA,利用Illumina Miseq测序平台对样本16S rRNA V3-V4区域进行测序,对测序结果进行生物信息学分析。结果Alpha多样性分析中,两组样本间Chao、observed species、Shannon及Simpson指数差异无统计学意义,但治疗后组均大于治疗前组。肠道菌群组成及结构差异分析显示门水平上两组所含优势菌群的相对丰度差异无统计学意义,而软壁菌门(Tenericutes)的相对丰度在经治疗后降低(P<0.05)。属水平上,治疗后组的瘤胃菌属、丁酸菌、克里斯滕森菌属_R-7_group、霍尔德曼氏菌属等菌属的相对丰度减少(P<0.05),而巴氏杆菌属、毛绒厌氧杆菌属及口腔杆菌属的相对丰度增加(P<0.05)。结论RA患者经^(99)Tc-MDP治疗前后的肠道菌群出现一定差异,且多样性有一定程度升高。Objective To investigate the changes of intestinal flora in patients with rheumatoid arthritis(RA)before and after treatment with technetium[^(99)Tc]methylenediphosphonate injection(^(99)Tc-MDP).Methods Thirty-two patients with RA were collected the fresh fecal specimens before and after they treated with ^(99)Tc-MDP.The genome DNA of fecal specimens was extracted and the V3-V4 regions of 16S rRNA were sequenced by Illumina Miseq platform.The sequencing results were analyzed by bioinformatics methodology.Results The Chao,observed species,Shannon and Simpson index between the two groups in Alpha diversity analysis had no statistical difference(P>0.05),but the value in post-treatment group were increased compared with the pre-treatment group.Compared with the pre-treatment of RA,there was no statistical difference in the higher proportion relative abundance on Phylum level of the intestinal microbes of RA patients,and the relative abundance of Tenericutes decreased(P<0.05).At the genus level,the relative abundance of Ruminococcaceae,Butyricicoccus,Christensenellaceae_R-7_group,Holdemania decreased after treatment(P<0.05).And the relative abundances of Pasteurella,Lachnoanaerobaculum and Stomatobaculum increased(P<0.05).Conclusion The abundance and species of gut microbiota in RA patients have some changes after treatment with ^(99)Tc-MDP,and the diversity increases after treatment.

关 键 词：类风湿关节炎 肠道菌群 锝[^(99)Tc]亚甲基二膦酸盐注射液 16S rRNA 

分 类 号：R593.22[医药卫生—内科学]