妊娠期高血压患者HLA-Ⅰ、miR-95-5p表达及与母婴不良结局相关性  

Expression of leukocyte antigen class I molecule and microrna-95-5p of women with gestational hypertension and their correlation with the maternal and infant outcomes

作　　者：刘丽丽[1] 刘静 Liu Lili;Liu Jing(Department of Obstetrics and Gynecology, Tangshan Maternal and Child Health Hospital Tangshan, Hebei 063000)

机构地区：[1]河北省唐山市妇幼保健院,063000

出　　处：《中国计划生育学杂志》2022年第1期172-176,共5页Chinese Journal of Family Planning

摘　　要：目的:分析妊娠期高血压患者血清白细胞抗原Ⅰ类分子(HLA-Ⅰ)、微小RNA-95-5p(miR-95-5p)表达及与母婴结局相关性。方法:选取2019年1月-2020年1月本院收治的妊娠期高血压患者95例为高血压组,产前检查正常妊娠孕妇80例为健康组,酶联免疫吸附测定法检测血清白细胞抗原-Ⅰ(sHLA-Ⅰ)水平,Western Blot法检测HLA-E、HLA-F、HLA-G蛋白水平,荧光定量PCR法检测血miR-95-5p表达量,统计并分析与各指标母婴结局关系。结果:与健康组相比,高血压组sHLA-Ⅰ、HLA-E、HLA-F、HLA-G表达降低,miR-95-5p表达升高(P<0.05)。HLA-Ⅰ、miR-95-5p表达与妊娠期高血压患者高血压家族史、妊娠高血压史有关(P<0.05)。高血压组不良母婴结局发生率高于健康组(P<0.05)。高血压组中,与正常母婴结局相比不良母婴结局者sHLA-Ⅰ、HLA-E、HLA-F、HLA-G表达降低,miR-95-5p表达升高(P<0.05)。sHLA-Ⅰ、HLA-E、HLA-F、HLA-G与miR-95-5p均呈负相关(P<0.05)。结论:妊娠期高血压患者血清HLA-Ⅰ类分子低表达、miR-95-5p高表达且与母婴不良结局相关,提示临床加以关注。Objective:To analyze the expression of leukocyte antigen class I(HLA-I)and microrna-95-5p(miR-95-5p)of women with gestational hypertension,and to study their correlation with the maternal and infant outcomes of the patients.Methods:95 women with gestational hypertension were selected in study group and 80 normal pregnant women who underwent physical examination were selected in control group from January 2019 to January 2020.The level of HLA-I of the women was detected by enzyme-linked immunosorbent assay(ELISA),and Western Blot was used to detect the protein level of HLA-E,HLA-F,and HLA-G of the patients.The expression of miR-95-5p in blood of the women was detected by RT-PCR.The correlation between the expressions of HLA-I,HLA-E,HLA-F,HLA-G,and miR-95-5p of the women and their maternal and infant outcomes was analyzed.Results:The expressions levels of HLA-I,HLA-E,HLA-F,and HLA-G of the women in the study group were significantly lower than those of the women in the control group,but the expression level of Mir-95-5P of the women in the study group was significantly higher(P<0.05).The expression levels of HLA-I and miR-95-5p of the women with gestational hypertension were correlated with their family history of hypertension and history of gestational hypertension(P<0.05).The incidence of adverse maternal and infant outcomes of the women in the study group was significantly higher than that of the women in the control group(P<0.05).In the women with gestational hypertension,the expression levels of HLA-I molecule of sHLA-I,HLA-E,HLA-F,and HLA-G of the women with adverse maternal and infant outcomes were significantly lower than those of the women with normal maternal and infant outcomes,but the expression of miR-95-5p of the women with adverse maternal and infant outcomes was significantly higher(P<0.05).The expression levels of sHLA-I,HLA-E,HLA-F,and HLA-G of the women were negatively correlated with their Mir-95-5p expression(P<0.05).Conclusion:HLA-I molecule of the women with gestational hypertension is low ex

关 键 词：妊娠期高血压 白细胞抗原Ⅰ类分子 微小RNA-95-5p 母婴结局 

分 类 号：R714.246[医药卫生—妇产科学]