丹红注射液对急性心肌梗死大鼠的治疗性血管新生作用研究  

Study on the effect of danhong injection in promoting therapeutic angiogenesis for acute myocardial infarction

作　　者：卫国 王慧芳 刘娟 刘迎宾 王艳华 殷英 Wei Guo;Wang Huifang;Liu Juan;Liu Yingbin;Wang Yanhua;Yinying(Dept of Pharmacy, The First Affiliated Hospital, Air Force Military Medical University, Xi’an 710032)

机构地区：[1]空军军医大学第一附属医院药剂科,西安710032

出　　处：《安徽医科大学学报》2022年第3期424-429,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81403135)。

摘　　要：目的明确丹红注射液对急性心肌梗死(AMI)大鼠的治疗性血管新生作用。方法40只大鼠随机分为假手术组、模型组、丹红注射液低(0.5 ml/kg)、中(1.0 ml/kg)、高(2.0 ml/kg)剂量组,每组8只。复制大鼠AMI模型,尾静脉注射法给药,连续给药7 d。超声心动图法测定大鼠左心室射血分数(LVEF)和左室短轴缩短率(LVFS),酶联免疫法检测大鼠血浆T型肌钙蛋白(TN-T)和脑钠肽(BNP)水平,HE染色法评价大鼠心肌损伤程度,免疫组化法测定大鼠心梗边缘区CD34阳性微血管密度(MVD),免疫印迹法测定大鼠心梗边缘区血管假性血友病因子(vWF)、α-平滑肌肌动蛋白(α-SMA)和血管内皮生长因子-A(VEGF-A)表达。结果与假手术组比较,模型组大鼠LVEF、LVFS值下降(P<0.01),TN-T和BNP水平升高(P<0.01),心梗边缘区α-SMA(P<0.05)、CD34、vWF、VEGF-A表达上调(P<0.01);与模型组比较,中、高剂量组LVEF、LVFS值上升,CD34、vWF表达增加(P<0.05,P<0.01),低、中、高剂量组TN-T和BNP水平下降,α-SMA、VEGF-A表达进一步增加(P<0.05,P<0.01);HE染色显示心肌组织病理损伤减轻。结论丹红注射液对AMI大鼠具有治疗性血管新生作用。Objective To confirm the therapeutic angiogenesis of danhong injection(DHI)in acute myocardial infarction(AMI)of rats.Methods Forty rats were randomly divided into five groups:Sham group,Model group,DHI low-dose(0.5 ml/kg)group,mid-dose(1.0 ml/kg)group,high-dose(2.0 ml/kg)group.The mouse model of AMI was induced by ligation of the left anterior descending coronary artery.Rats were treated with different doses of DHI(0.5,1.0,2.0 ml/kg)for 7 days.Cardiac function of rats was indicated by left ventricular ejection fraction(LVEF)and fractional shortening(LVFS),serum levels of troponin T(TN-T)and brain natriuretic peptide(BNP),myocardial pathological damage,CD34 positive microvessel density(MVD),the expression of Von willebr and factor(vWF),alpha-smooth muscle actin(α-SMA)and vascular endothelial growth factor-A(VEGF-A)were measured by echocardiography,enzyme linked immunosorbent assay,HE staining,immunohistochemistry and Western blot,respectively.Results Compared with Sham group,LVEF,LVFS in Model group increased(P<0.01),serum levels of TN-T and BNP decreased(P<0.01),α-SMA(P<0.05),CD34,vWF,VEGF-A(P<0.01)were up-regulated.Compared with Model group,LVEF,LVFS and the expression of CD34,vWF in mid-dose and high-dose groups increased(P<0.05,P<0.01).TN-T,BNP in all dosage groups were reduced while the expression ofα-SMA,VEGF-A were further upgraded(P<0.05,P<0.01).Besides,the pathological damage in the marginal zone of infarcted myocardium was lessened.Conclusion DHI has an effect on therapeutic angiogenesis for AMI in rats.

关 键 词：丹红注射液 急性心肌梗死 治疗性血管新生 

分 类 号：R256.2[医药卫生—中医内科学] R285.5[医药卫生—中医学]