延龄草皂苷通过降低成纤维细胞活化蛋白表达水平抑制人类结肠癌细胞增殖、凋亡、迁移和侵袭  

Inhibit of Trillium saponins on the proliferation,apoptosis,migration and invasion of human colon cancer cells by reducing the level of fibroblast activation protein expression

作　　者：周峰[1] 刘红[1] 陈维 ZHOU Feng;LIU Hong;CHEN Wei(Department of Clinical Surgery,Rugcw People's Hospital,Rugao 214516,Jiangsu Province’China;Department of Clinical Chemotherapy,Rugcw People's Hospital,Rugao 214516,Jiangsu Province’China)

机构地区：[1]南通大学附属如皋医院、江苏省如皋市人民医院普通外科,江苏如皋214516 [2]南通大学附属如皋医院、江苏省如皋市人民医院临床化疗科,江苏如皋214516

出　　处：《中国临床药理学杂志》2022年第5期391-394,共4页The Chinese Journal of Clinical Pharmacology

基　　金：南通市卫生健康委员会面上课题基金资助项目(MB2021085)。

摘　　要：目的研究延龄草皂苷是否能通过影响纤维细胞活化蛋白(FAP)表达来抑制结肠癌细胞的增殖、凋亡、迁移和侵袭。方法将对数生长期结肠癌细胞HCT116分为5组:对照组、siRNA组、低剂量实验组、中剂量实验组和高剂量实验组。低、中、高剂量实验组分别用含浓度为30, 60, 90 mg·L^(-1)延龄草皂苷的DMEM培养基100μL进行培养,对照组用等剂量的1%甲醇溶剂DMEM培养基进行培养,siRNA组用等剂量siRNA试剂且不含药物的DMEM培养基进行培养。用四唑盐(MTT)比色法增殖细胞实验筛选半数抑制浓度(IC_(50))及药物干预浓度;用实时荧光定量多聚核苷酸链式反应分析检测FAP基因表达水平;用MTT比色法检测细胞增殖情况;用流式细胞术检测细胞凋亡情况;用Transwell实验检测细胞迁移和侵袭情况。结果对照组、siRNA组、低剂量实验组、中剂量实验组和高剂量实验组的细胞增殖抑制率分别为(0.00±0.01)%,(55.03±6.32)%,(12.63±1.82)%,(28.92±2.48)%和(46.22±5.01)%;这5组的FAP基因表达分别为1.00±0.01,0.01±0.01,0.42±0.13,0.12±0.06和0.03±0.05;这5组的细胞凋亡率分别为(5.02±0.92)%,(68.92±7.01)%,(18.02±2.11)%,(35.22±4.67)和(60.18±6.33)%;这5组的细胞迁移水平分别为150.67±16.24,21.97±1.63,90.27±9.33,65.20±5.31和41.37±3.46;这5组的细胞侵袭水平分别为139.00±13.52,12.40±1.00,87.17±8.00,52.43±4.20和35.63±2.18。上述指标,实验组与对照组比较,差异均具有统计学意义(均P<0.05)。结论延龄草皂苷通过降低FAP表达水平抑制人类结肠癌细胞增殖、凋亡、迁移和侵袭。ObjectiveTo investigate whether fibroblast activation protein (FAP) expression can inhibit the proliferation,apoptosis,migration and invasion of colon cancer cells.Methods Logarithmic growth phase colon cancer cells HCT116 were divided into five groups:control group,siRNA group,experimental-L group,experimental-M group and experimental-H group.The experimental-L group,experimental-M group and experimental-H group were incubated with100μL of DMEM medium containing 30,60 and 90 mg·L^(-1) of Trillium saponins,respectively;the control group was incubated with an equal dose of 1%methanol solvent DMEM medium;the siRNA group was incubated with an equal dose of siRNA reagent and drug-free DMEM medium.The cell proliferation assay was performed by tetrazolium salt (MTT) colorimetric assay to screen the IC_(50) and drug intervention concentrations;real-time fluorescence analysis to detect FAP mRNA expression;MTT colorimetric assay to detect cell proliferation;flow cytometry to detect apoptosis;Transwell assay to detect cell migration and invasion;cell migration and invasion were detected by Transwell assay.Results The inhibition rates of cell value addition in the control,siRNA,experimental-L group,experimental-M group and experimental-H group were (0.00±0.01)%,(55.03±6.32)%,(12.63±1.82)%,(28.92±2.48)%and (46.22±5.01)%;FAP mRNA expression of these five groups were 1.00±0.01,0.01±0.01,0.42±0.13,0.12±0.06 and 0.03±0.05,respectively;the apoptosis rates of these five groups were (5.02±0.92)%,(68.92±7.01)%,(18.02±2.11)%,(35.22±4.67)%and(60.18±6.33)%;the cell migration levels of these five groups were 150.67±16.24,21.97±1.63,90.27±9.33,65.20±5.31 and 41.37±3.46,respectively;the cell The levels of invasion in these five groups were139.00±13.52,12.40±1.00,87.17±8.00,52.43±4.20 and 35.63±2.18,respectively.The differences between the above indicators in the experimental group and the control group were all statistically significant (all P<0.05).Conclusion Trillium saponins inhibited the proliferation,apopto

关 键 词：延龄草皂苷 结肠癌细胞株HCT116 成纤维细胞活化蛋白 增殖 凋亡 迁移 侵袭 

分 类 号：R979.1[医药卫生—药品]