芍药苷对伴刀豆球蛋白诱导的小鼠急性肝损伤的保护作用  

Protective effect of paeoniflorin on acute liver injury induced by concanavalin A in mice

作　　者：李聪 周威 LI Cong;ZHOU Wei(General surgery department,Xuancheng People's Hospital,Xuancheng 242000,Anhui Province,China)

机构地区：[1]宣城市人民医院普外科,安徽宣城242000

出　　处：《中国临床药理学杂志》2022年第5期427-431,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探究芍药苷在伴刀豆球蛋白(Con A)诱导的小鼠急性肝损伤中的作用。方法通过尾静脉注射20 mg·kg^(-1)的ConA得到小鼠急性肝损伤模型。将小鼠随机分成空白组、模型组、低剂量实验组、高剂量实验组。低剂量实验组、高剂量实验组每日灌胃40和100 mg·kg^(-1)芍药苷,连续7 d;空白组、模型组在相同时间点给予等量生理盐水灌胃。最后1次灌胃后模型组、低剂量实验组、高剂量实验组均进行造模处理。以全自动生化分析仪检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)和碱性磷酸酶(ALP)活力;以酶联免疫吸附测定法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平;用蛋白质印迹法检测丝裂原活化蛋白激酶(MAPK)、磷酸化的丝裂原活化蛋白激酶(p-MAPK3)、信号转导和转录激活因子(STAT3)、磷酸化的信号转导和转录激活因子3(p-STAT3)蛋白表达情况。结果空白组、模型组、低剂量实验组、高剂量实验组的GPT活力分别为(40.56±3.36),(201.48±5.05),(157.16±7.71)和(141.95±3.87) U·L^(-1),这4组的TNF-α水平分别为(43.62±2.87),(541.60±36.03),(407.05±24.26)和(349.56±24.28) pg·mL^(-1);这4组的p-MAPK/MAPK表达水平分别为1.00±0.06,2.97±0.12,2.16±0.09和1.45±0.11;这4组的p-STAT3/STAT3表达水平分别为1.00±0.04,1.98±0.20,1.62±0.01和1.04±0.02。上述指标,模型组与空白组比较,差异均有统计学意义(均P<0.05);低、高剂量实验组与模型组比较,差异均有统计学意义(均P<0.05)。芍药苷可以降低Con A诱导的小鼠急性肝损伤小鼠肝系数,脾系数和胸腺系数。结论芍药苷通过影响MAPK/STAT3信号通路改善Con A诱导的小鼠急性肝损伤。Objective To investigate the effect of paeoniflorin on acute liver injury induced by concanavalin A (Con A) in mice.Methods Acute liver injury model of mice was established by injection of 20mg·kg^(-1) Con A into the tail vein.The mice were randomly divided into normal group,model group,experimental-L group and experimental-H group.The experimental-L group and experimental-H group were given 40 and 100 mg·kg^(-1) paeoniflorin every day for 7 consecutive days.The normal group and model group were given the same amount of normal saline at the same time point.After the last gavage infusion,the model group,experimental-L group and experimental-H group were all treated with acute liver injury model.The activities of glutamic-pyruvic transaminase (GPT),glutamicoxaloacetic transaminase (GOT) and alkaline phosphatase (ALP) in serum were detected by automatic biochemical analyzer;the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in serum were detected by enzyme-linked immunosorbent assay;the levels of mitogen activated protein kinase (MAPK),phosphorylated mitogen activated protein kinase (p-MAPK),signal transduction and transcription activator 3 (STAT3) and phosphorylated signal transduction and transcription activator 3 (p-STAT3) were detected by westernblotting.Results GPT activity of normal group,model group,experimental-L group and experimental-H group were (40.56±3.36),(201.48±5.05),(157.16±7.71) and (141.95±3.87) U·L^(-1),respectively;TNF-αlevels were(43.62±2.87),(541.60±36.03),(407.05±24.26) and (349.56±24.28) pg·m L^(-1),respectively;pmapk/MAPK expression levels were 1.00±0.06,2.97±0.12,2.16±0.09 and 1.45±0.11,respectively;p-stat3/STAT3 expression levels were 1.00±0.04,1.98±0.20,1.62±0.01 and 1.04±0.02,respectively.Comparison between model group and normal group of the above indicators,the difference was significantly (all P<0.05);Comparison between experimental-L group,experimental-H group and model group of the above indicators,the difference was significantly (all

关 键 词：芍药苷 丝裂原活化蛋白激酶/信号转导和转录激活因子3信号通路 急性肝损伤 伴刀豆球蛋白 

分 类 号：R97[医药卫生—药品]