转基因抗虫、耐除草剂及品质改良复合性状玉米BBHTL8-1的分子特征及功能评价  

Molecular Characterizations of Stacked Transgenic Maize BBHTL8-1 with Insect Resistance,Glyphosate Tolerance and Improved Quality

作　　者：邹俊杰 徐妙云[1,2] 张兰[1] 罗彦忠[1] 刘源 郑红艳[1] 王磊 ZOU Junjie;XU Miaoyun;ZHANG Lan;LUO Yanzhong;LIU Yuan;ZHENG Hongyan;WANG Lei(Key Laboratory of Agricultural Genomics(Beijing),Ministry of Agriculture and Rural Affairs,Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;National Nanfan Research Institute(Sanya),CAAS,Hainan Sanya 572000,China)

机构地区：[1]中国农业科学院生物技术研究所,农业农村部农业基因组学重点实验室(北京),北京100081 [2]三亚中国农业科学院国家南繁研究院,海南三亚572000

出　　处：《中国农业科技导报》2022年第2期77-85,共9页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种培育重大专项(2018ZX08003-03B);海南三亚崖州湾科技城管理局科研项目(SKJC-2020-02-005)。

摘　　要：随着转基因作物的发展和推广,具有复合性状的转基因作物种植面积逐年增加。传统的转基因复合性状作物具有抗虫和耐除草剂性能,聚合其他性状的转基因作物培育逐渐受到重视。转基因玉米株系BBHTL8-1含有Cry1Ab、Cry3Bb、cp4epsps、ZmHPT和ZmTMT共5个基因表达框,外源插入片段位于玉米基因组第4染色体,插入基因区间,在3’侧翼序列有31 bp缺失。在BBHTL8-1中,这5个基因能够稳定遗传,在转录水平和蛋白水平上表达具有稳定性。BBHTL8-1高抗玉米螟,对草甘膦除草剂具有较高耐受性。BBHTL8-1籽粒中α-生育酚含量比非转基因对照郑58显著增加。BBHTL8-1与非转基因对照郑58在田间农艺性状方面无显著差异。因此,BBHTL8-1为转基因抗虫、耐除草剂和种子具有高α-生育酚含量的转基因新材料。With the development of genetically modified crops,transgenic plants containing stacked traits become more and more popular.Transgenic maize plants with stacked traits are usually insect resistance and herbicide tolerance.Here,BBHTL8-1 transgenic maize line containing Cry1Ab,Cry3Bb,cp4epsps,ZmHPT and ZmTMT expressing cassettes was obtained to confer transgenic maize plants with insect resistance,glyphosate tolerance and improvedα-tocopherol content traits.In BBHTL8-1 transgenic line,all 5 genes were integrated in the maize genome and could be identified in different generations.Whole genome sequencing and flanking sequence analysis showed that the insertion fragment was located in the intergenic region of chromosome 4,with 31 bp deletion in the 3’flank sequence.Cry1Ab,Cry3Bb and cp4epsps showed relatively stable expression levels in different development stages of root,stem and leaf,and had lower expression levels in the seeds,whereas ZmHPT and ZmTMT had higher expression levels in the seeds.Evaluations of insect resistance and glyphosate tolerance revealed that BBHTL8-1 plants were highly resistant to Asian corn borer,and were highly tolerant to glyphosate.Theα-tocopherol content in BBHTL8-1 seeds was significantly higher than in non-transgenic plants Zheng 58.There were no significant differences between BBHTL8-1 and Zheng 58 on agronomic traits under normal growth condition.Above results obtained a new transgenic maize line with stacked traits on insect resistance,herbicide tolerance and nutritional quality improvement,which might be developed for transgenic breeding.

关 键 词：转基因玉米 复合性状 抗虫 耐除草剂 Α-生育酚 分子特征 

分 类 号：S513[农业科学—作物学] Q78[生物学—分子生物学]