淫羊藿次苷Ⅱ对脑缺血/再灌注损伤大鼠脑细胞凋亡和自噬的影响  

Effects of icarisideⅡon brain cell apoptosis and autophagy in rats with cerebral ischemia/reperfusion injury

作　　者：刘禹岐 郗欧 刘怡彤 LIU Yu-qi;XI Ou;LIU Yi-tong(No.1 Departmen of Encephalopathy,the Second Affiliated Hospital of Liaoning University of Chinese Medicine,Shenyang 110000;Department of Rehabilitation,the Second Affiliated Hospital of Liaoning University of Chinese Medicine,Liaoning 110000)

机构地区：[1]辽宁中医药大学附属第二医院脑病一科,辽宁沈阳110000 [2]辽宁中医药大学附属第二医院康复科,辽宁沈阳110000

出　　处：《湖北中医药大学学报》2022年第1期53-57,共5页Journal of Hubei University of Chinese Medicine

基　　金：辽宁省中医药管理局科研项目(项目编号:LNZYXZK201906)。

摘　　要：目的观察淫羊藿次苷Ⅱ对脑缺血/再灌注损伤大鼠脑细胞凋亡和自噬的影响。方法将50只SPF级大鼠分为对照组、模型组、低剂量组、中剂量组、高剂量组,每组10只,其中对照组、模型组灌胃等量生理盐水,低剂量组、中剂量组、高剂量组给与灌胃淫羊藿次苷Ⅱ为4、8、16mg/kg。给药7d后除对照组外,其余4组用大脑中动脉线栓法建立脑缺血/再灌注损伤模型。24h后处死大鼠,比较各组大鼠神经功能评分,计算脑组织含水量;采用TTC法检测大鼠脑梗死体积;采用流式细胞术检测大鼠脑细胞凋亡;Western blot检测大鼠脑组织Bcl-2、Bax、cleaved-caspase9、LC3Ⅱ/LC3I、Beclin1、p62蛋白表达;ELISA检测血清IL-1β、TNF-α的表达。结果与对照组相比,模型组的神经功能评分、脑组织含水量、脑梗死体积、Bax、cleaved-caspase9、LC3Ⅱ/LC3I、Beclin1蛋白表达、IL-1β、TNF-α表达明显升高;Bcl-2、p62蛋白表达明显降低。与模型组相比,低剂量组、中剂量组、高剂量组的神经功能评分、脑组织含水量、脑梗死体积、Bax、cleaved-caspase9、LC3Ⅱ/LC3I、Beclin1蛋白表达、IL-1β、TNF-α表达明显降低;Bcl-2、p62蛋白表达明显升高。结论淫羊藿次苷Ⅱ能减轻脑缺血/再灌注炎性损伤,减少细胞凋亡和自噬。Objective To investigate the effect of icarisideⅡon brain cell apoptosis and autophagy in rats with cerebral ischemia/reperfusion injury.Methods Fifty SPF rats were divided into control group,model group,icarisideⅡlow-dose,medium-dose,and high-dose groups,10 in each.Control group and model group were given the same amount of normal saline,and icarisideⅡlow-dose,middle-dose and high-dose groups were given 4,8,16 mg/kg of icarisideⅡ.Except for control group,the other four groups were treated with middle cerebral artery suture method to establish cerebral ischemia/reperfusion injury models after 7 d of administration.Rats were sacrificed after 24 h,the neurological function scores of the rats in each group were compared,the cerebral infarction volume and brain tissue water content were calculated;TTC method was used to detect cerebral infarct volume in rats;the rat brain cell apoptosis was detected by flow cytometry;Western blot was used to detect Bcl-2,Bax,cleaved-caspase9,LC3Ⅱ/LC3 I,Beclin1,p62 protein expression in rat brain tissue;ELISA was used to detect serum interluelcin-1(IL-1β)and tumor necrons factor-(TNF-α)expression.Results Compared with control group,the neural function,brain water content,cerebral infarction volume,Bax,cleaved-caspase9,LC3Ⅱ/LC3 I,Beclin1 protein expression,IL-1β,TNF-αexpression in model group were significantly increased;the expression of Bcl-2 and p62 protein decreased significantly.Compared with model group,the neurological function,brain water content,cerebral infarction volume,Bax,cleaved-caspase9,LC3Ⅱ/LC3 I,Beclin1 protein expression,IL-1βand TNF-αexpression in icarisideⅡlow-dose,middle-dose and high-dose groups were significantly reduced,while the expression of Bcl-2 and p62 protein were significantly increased.Conclusion IcarisideⅡcan relieve cerebral ischemia/reperfusion inflammatory injury,reduce cell apoptosis and autophagy.

关 键 词：淫羊藿次苷Ⅱ 脑缺血/再灌注 凋亡 自噬 

分 类 号：R285.5[医药卫生—中药学]