黑龙江省汉族人群新生儿缺氧缺血性脑病miRNA-210表达变化的研究  

Study on the expression of miRNA-210 in neonates with hypoxic ischemic encephalopathy in Han nationality of Heilongjiang province

作　　者：张琳 李玥[2] 刘春阳 刘宁 徐辉[2] ZHANG Lin;LI Yue;LIU Chun yang;LIU Ning;XU Hui(Clinical Medical School of Jiamusi University,Jiamusi 154007,China;Basic Medical School of Jiamusi University,Jiamusi 154007,China;Medical School of Ankang University,Ankang 725000,China)

机构地区：[1]佳木斯大学临床医学院,黑龙江佳木斯154007 [2]佳木斯大学基础医学院,黑龙江佳木斯154007 [3]安康学院医学院,陕西安康725000

出　　处：《黑龙江医药科学》2022年第1期1-3,共3页Heilongjiang Medicine and Pharmacy

基　　金：国家自然科学基金,编号:81273174;黑龙江省省属高等学校基本科研业务费项目,编号:2019-KYYWF-1337;黑龙江省大学生创新创业训练计划项目,编号:201910222062;黑龙江省自然科学基金,编号:LH2020H007。

摘　　要：目的:观察黑龙江省汉族人群HIE患儿血清miRNA-210的表达变化,探讨miRNA-210在新生儿脑缺氧缺血后可能发挥的作用。方法:采集静脉血取血清,应用qRT-PCR检测比较各实验组miRNA-210的表达水平,ELISA定量测定血清中HIF-1α含量。结果:病例组样本中miRNA-210的表达水平高于对照组(F=106.41,P<0.01),HIF-1α含量明显升高(F=29.63,P<0.01),与对照组的差异有统计学意义。结论:HIE血清样本中miRNA-210的表达水平上调,HIF-1α含量明显升高,miRNA-210可能在HIE中发挥重要作用。Objective:To observe the changes of serum miRNA-210 expression in children with HIE in Han nationality in Heilongjiang Province,and to explore the possible role of miRNA-210 in neonatal cerebral hypoxia-ischemia.Methods:Serum was collected from venous blood,the expression levels of miRNA-210 in each experimental group were compared by qRT-PCR.and the levels of HIF-1αin serum were determined by ELISA.Results:The expression level of miRNA-210 in the sample group was higher than that in the control group(F=106.41,P<0.01),and the levels of HIF-1αwere significantly increased(F=29.63,P<0.01).The difference was statistically significant from the control group.Conclusion:The expression level of miRNA-210 is up-regulated in HIE serum samples,and the levels of HIF-1αare significantly increased.miRNA-210 may play an important role in HIE.

关 键 词：新生儿缺氧缺血性脑病 miRNA-210 缺氧诱导因子-1 

分 类 号：R722.1[医药卫生—儿科]