Pachymic Acid Ameliorates Pulmonary Hypertension by Regulating Nrf2-Keap1-ARE Pathway  

作　　者：Yuan HE Jian-hua ZHONG Xiao-dong WEI Chu-ying HUANG Pai-lan PENG Jun YAO Xiu-sheng SONG Wan-li FAN Guang-cai LI 

机构地区：[1]Department of Ultrasound,the Central Hospital ofEnshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China [2]Department of Neurology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China [3]Department of Thoracic and Cardiovascular Surgery,Minda Hospital of Hubei Minzu University,Enshi 445000,China [4]Department of Pulmonary and Critical Care Medicine,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China

出　　处：《Current Medical Science》2022年第1期56-67,共12页当代医学科学（英文）

基　　金：This project was supported by the Natural Science Foundation of Hubei Province(No.2017CFB769).

摘　　要：Objective:Pulmonary hypertension(PH)is a severe pulmonary vascular disease that eventually leads to right ventricular failure and death.The purpose of this study was to investigate the mechanism by which pachymic acid(PA)pretreatment affects PH and pulmonary vascular remodeling in rats.Methods:PH was induced via hypoxia exposure and administration of PA(5 mg/kg per day)in male Sprague-Dawley rats.Hemodynamic parameters were measured using a right ventricular floating catheter and pulmonary vascular morphometry was measured by hematoxylin-eosin(HE),a-SMA and Masson staining.MTT assays and EdU staining were used to detect cell proliferation,and apoptosis was analyzed by TUNEL staining.Western blotting and immunohistochemistry were used to detect the expression of proteins related to the Nrf2-Keapl-ARE pathway.

关 键 词：pulmonary hypertension pachymic acid nuclear factor erythroid 2-related factor 2 Kelch-like epichlorohydrin-related protein 1 antioxidant response element 

分 类 号：R54[医药卫生—心血管疾病]