Characterization and Optimization of Amylase Production in Strains LZ-10 and LZ-11 Belonging to Bacillus subtilis  

作　　者：Zhao Shu-qin Yang Xiao-pu Gao Yuan 

机构地区：[1]Foundation Experiment Teaching Center,Gansu Agricultural University,Lanzhou 730070,China [2]College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2022年第1期79-88,共10页东北农业大学学报（英文版）

基　　金：Supported by Young Doctor Fund of Gansu Education Department,China(2021QB-034)。

摘　　要：In order to obtain pure enzyme with high activity,two amylase producing strains were isolated from soil samples,and named as the strains LZ-10 and LZ-11.According to morphologic observation,physiology and biochemistry experiments,16S rRNA and gyrB gene analysis,the strains LZ-10 and LZ-11 were identified as Bacillus subtilis.Adopted the method of ammonium sulfate,DEAE-52 anion purify enzyme,finally used polyacrylamide gel electrophoresis(SDS-PAGE)to detect molecular weight.The strain LZ-10 had an amylase activity of 123.3 U·mL^(-1),a purification factor of 6.8,a recovery rate of 69.5% and an optimal temperature of 50℃.The amylase activity of the strain LZ-11 was 59.91 U·mL^(-1),the purification factor was 4.5,the recovery rate was 60.5%,and the optimum temperature was 55℃.The commodity enzyme derived from Bacillus subtilis was 37.5 U·mL^(-1).The relative molecular weight of amylase activity from the two strains was 55 ku.Both thermal stability and pH stability were higher than those of commercialized amylase.

关 键 词：AMYLASE SCREENING enzyme purification enzymatic property 

分 类 号：Q815[生物学—生物工程]