五步蛇蛇毒化学成分分析及其抗肿瘤活性筛选研究  

Analysis of the Chemical Constituents of Agkistrodon Acutus Venom and Their Antitumor Activity Screening

作　　者：曾晓艳[1] 郑沛 曹玮龙 龚力民[1] 刘塔斯[1] 刘应蛟 Zeng Xiaoyan;Zheng Pei;Cao Weilong;Gong Limin;Liu Tasi;Liu Yingjiao(School of Pharmacy,Hunan University of Chi-nese Medicine,Changsha 410000,China;School of Pharmacy,Jiangxi University of Chinese Medicine)

机构地区：[1]湖南中医药大学药学院,长沙410000 [2]江西中医药大学药学院

出　　处：《中国药师》2022年第3期439-444,共6页China Pharmacist

基　　金：湖南省自然科学基金项目(编号:2019JJ50435);2021年中央财政国家重点野生动植物保护资金资助项目(编号:2130211)。

摘　　要：目的:对五步蛇蛇毒进行化学成分分析及其抗肿瘤生物活性研究,为蛇毒成分在抗肿瘤领域的进一步开发提供参考。方法:采用考马斯亮蓝法测定五步蛇蛇毒总蛋白含量,SDS-PAGE法分析蛇毒蛋白成分;以肝癌细胞Hep G2、乳腺癌细胞MDA-MB-231为研究对象,采用MTT法对蛇毒抗肿瘤活性进行筛选。结果:蛇毒总蛋白平均含量为65.6%;蛇毒蛋白质相对分子量测定得到9条蛋白条带;随着蛇毒浓度的增加,对Hep G2细胞和MDA-MB-231细胞的抑瘤作用越大,且呈浓度依赖性。结论:该方法为蛋白质纯化、蛋白质生物活性、结构与功能的研究提供实验数据,为五步蛇蛇毒蛋白资源进一步深层次研究和利用提供依据,同时为蛇毒抗肿瘤活性研究提供科学参考。Objective: To analyze the chemical constituents of agkistrodon acutus venom and research their anti-tumor biological activity to provide reference for the research and development of agkistrodon acutus venom in the anti-tumor field.Methods: Coomasius bright blue method was used to determine the total protein content of agkistrodon acutus venom, and SDS-PAGE method was used to analyze the components of agkistrodon acutus venom. HepG2 and MDA-MB-231 were selected as the research objects, and MTT assay was used to screen the anti-tumor activity of agkistrodon acutus venom. Results: The total protein content of agkistrodon acutus venom was 65.6%. The relative molecular weight of snake venom protein was determined and nine protein bands were found. With the increase of agkistrodon acutus venom concentration, the anti-tumor effects of agkistrodon acutus venom on Hep G2 cells and A-MB-231 cells increased in a concentration-dependent manner. Conclusion: The method provides experimental data for the study of protein purification, biological activity, structure and function, and provides basis for further research and utilization of agkistrodon acutus venom protein resources. It also provides scientific reference for the study of anti-tumor activity of agkistrodon acutus venom.

关 键 词：蛇毒 含量测定 紫外分光光度计 聚丙烯酰胺凝胶电泳法 人肝癌细胞 乳腺癌细胞 MTT比色法 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]